A cationic polymer that responds to decharge by oxidation, its preparation method and application
A technology of cationic polymers and compounds, applied in other methods of inserting foreign genetic materials, medical preparations of non-active ingredients, drug combinations, etc., can solve undisclosed problems and achieve convenient preparation and strong lysosome escape ability , the effect of simple structure
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0049] The synthesis of embodiment 1, B-PIEMA
[0050]
[0051]1-(2-Hydroxyethyl)imidazole (5.0g, 44.59mmol) was dissolved in dry dichloromethane and placed in an ice-water bath, and methacryloyl chloride (5.6g, 53.57mmol) was dissolved in dry dichloromethane Chloromethane was slowly added dropwise using a constant pressure dropping funnel. After the dropwise addition was completed, the ice-water bath was removed and the temperature was raised slowly, and the reaction was carried out overnight at room temperature. After the completion of the reaction as detected by TLC, the reaction solution was diluted with dichloromethane, washed three times with saturated aqueous sodium bicarbonate solution and saturated saline, the organic phase was collected, dried over anhydrous sodium sulfate, the filtrate was collected by filtration, and the organic solvent was removed by rotary evaporation. The crude product was separated and purified by silica gel column (ethyl acetate / n-hexane) ...
Embodiment 2
[0054] The NMR characterization of embodiment 2, B-PIEMA
[0055] The proton NMR characterization of B-PIEMA is as follows: 1 H-NMR,D 2 O: δ=8.7-9.1(1H, -NCHN-), δ=7.2-8.2(7H, ArH, -NCHCHN-), δ=5.4-5.8(2H, ArHCH 2 N-),δ=4.2-4.7(4H,-NCH 2 CH 2 OOCCH-),δ=2.3-2.6(1H,-CH 2 CH-),δ=1.6-2.2(-CH 2 CH-),δ=0.8-1.4(CH 3 -).
Embodiment 3
[0056] Example 3, B-PIEMA charge change under oxidation conditions
[0057] A certain amount of B-PIEMA was weighed and dissolved in HEPES buffer solution (pH 7.4, 10mM) at a concentration of 3mg / mL, and the final concentration of hydrogen peroxide solution was added to 80mM. Always maintain the pH value of the solution at 7.4, incubate at 37°C, and measure the Zeta potential of the solution at different times. Test results such as figure 1 As shown, the Zeta potential of B-PIEMA dropped from 30mV to 20mV quickly, and then the charge decreased slowly with time, and could drop to 10mv in 30h.
[0058] Experiments have proved that the charge of B-PIEMA will change under the treatment of active oxygen, thereby reducing the force with DNA and releasing DNA.
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information

- R&D
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com