Device for animal muscle cell culture

Abstract

The invention provides a device for animal muscle cell culture, and belongs to the technical field of cell culture. According to the technical scheme, the device for animal muscle cell culture is characterized by comprising a culture dish body and a dish cover, a sealed cavity is formed in the lower portion of the culture dish body, and a liquid inlet pipe communicated with the cavity is arrangedin the culture dish body; a liquid outlet of the liquid inlet pipe is higher than the height of the liquid level in the culture dish body and the height of the liquid level in the cavity; the device further comprises a container with an adjustable liquid storage space, the container is communicated with the cavity through a first one-way valve, and the liquid storage space of the container becomessmaller so that liquid in the container can enter the culture dish body through the sealed cavity and the liquid inlet pipe. The device has the beneficial effects that the culture dish can be assisted in manually adding the culture solution into the culture dish under the condition of not moving the culture dish and opening the cover.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a device for culturing animal muscle cells. Background technique [0002] Cell culture usually uses culture flasks or petri dishes. Due to the small diameter of the bottle mouth of the culture bottle, it is not easy to handle when inoculating tissue pellets in primary culture. The most commonly used at present is the petri dish, and the petri dish has the following disadvantages: one is that the lid is loosely covered on the petri dish, and the lid will be moved accidentally when taking the petri dish, causing bacteria to grow in the culture solution; The side wall is not high, and when you take it, the movement range is slightly to make the culture solution shake to the cover, which will also cause bacteria to grow in the culture solution, which is not conducive to microscope observation. [0003] In the primary culture, after the tissue particles to be inoculated adhere ...

AI Technical Summary

Problems solved by technology

Due to the small diameter of the bottle mouth of the culture bottle, it is not easy to handle when inoculating tissue pellets in primary culture

The most commonly used at present is the petri dish, and the petri dish has the following disadvantages: one is that the lid is loosely covered on the petri dish, and the lid will be moved accidentally when taking the petri dish, causing bacteria to grow in the culture solution; The side wall is not high, and when you take it, the movement range is slightly to make the culture solution shake on the cover, which will also cause bacteria to grow in the culture solution, which is not conducive to microscope observation

During the entire moving process, in order to avoid the growth of bacteria in the culture medium, the operator will be very careful and meticulous in the operation, which is time-consuming and laborious

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