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Peripheral blood miRNA colon cancer diagnostic marker combination and detection kit thereof

A detection kit and detection reagent technology, applied in the fields of biomedical technology and molecular biology, can solve problems such as differences, and achieve the effects of less blood sample volume, high sensitivity and high throughput

Active Publication Date: 2021-04-06
中国医科大学
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  • Abstract
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  • Claims
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Problems solved by technology

Tumor-derived miRNAs are derived from the passive release and active efflux of secretory bodies after cancer tissue damage, which leads to significant differences in the expression profile of miRNAs in peripheral blood of tumor populations compared with healthy populations

Method used

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  • Peripheral blood miRNA colon cancer diagnostic marker combination and detection kit thereof
  • Peripheral blood miRNA colon cancer diagnostic marker combination and detection kit thereof
  • Peripheral blood miRNA colon cancer diagnostic marker combination and detection kit thereof

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Embodiment Construction

[0027] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention and the accompanying drawings. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention. The reagents involved in the examples of the present invention are all commercially available products, and can be purchased through commercial channels unless otherwise specified. In some embodiments, the present invention uses universal reverse transcription primers and qPCR universal reverse primers, which are commercially available from Quan Shi Jin Company.

[0028] A peripheral blood miRNA marker combination for colon cancer diagnosis, said miRNA marker combination ...

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Abstract

The invention relates to the field of biomedical technologies and molecular biology, in particular to a peripheral blood miRNA marker combination and kit for diagnosing colon cancer and application of the peripheral blood miRNA marker combination and kit. The invention discloses a peripheral blood miRNA marker combination for colon cancer diagnosis. The miRNA marker combination comprises one or a combination of at least two of the following miRNAs: hsa-miR-1324, hsa-miR-20b-5p, hsa-miR-216b-5p, hsa-miR-367-5p, hsa-miR-597-5p and hsa-miR-934. The peripheral blood miRNA marker combination for colon cancer diagnosis is applied to preparation of a colon cancer detection reagent or tool. The peripheral blood miRNA tumor marker detection provided by the invention belongs to the field of liquid biopsy, has the characteristics of high specificity, earlier discovery, high sensitivity and minimal invasion compared with clinically conventional blood tumor markers, and is a novel marker for diagnosing various diseases including tumors. The detection result of the training set established by the invention on the test set shows that the diagnosis efficiency is as high as 95%, and the method has important significance for clinical colon cancer diagnosis in the future.

Description

technical field [0001] The invention relates to the fields of biomedical technology and molecular biology, in particular to a combination of peripheral blood miRNA markers for diagnosing colon cancer, a kit and applications thereof. Background technique [0002] Colon cancer is one of the common clinical malignant tumors, and its morbidity and mortality rate ranks third among malignant tumors in the world. The 2018 China Cancer Statistics Report shows that the incidence of colon cancer in my country ranks third among all malignant tumors, with 376,000 new cases and 191,000 deaths. Among them, cities are much higher than rural areas, and the incidence of colon cancer has increased significantly. The authoritative medical journal CA report predicts that in 2020 in China, the incidence and mortality of colon cancer will maintain an upward trend. [0003] Because the clinical manifestations of colon cancer are not obvious in the early stage, many patients have developed to the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/158C12Q2600/178C12Q2600/166
Inventor 张晶魏敏杰江龙洋卫倩毕佳张振孟喆昊赵小倩
Owner 中国医科大学
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