A method for detecting cell viability based on delayed luminescence kinetics
A technology of delayed luminescence and viability detection, which is applied in the fields of informatics, bioinformatics, material excitation analysis, etc., and can solve the problems of destroying cell structure, cells being unable to continue to be cultured or applied, and death
Active Publication Date: 2021-12-14
TIANJIN POLYTECHNIC UNIV +1
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Problems solved by technology
However, these methods are often invasive, and the biochemical reactions, staining, labeling and other treatment methods used in them will change the normal growth environment and physiological functions of cells, and even destroy the cell structure, causing irreversible cell damage and death
In addition, these methods have high requirements for experimental operation techniques, and the cells after detection cannot be further cultured or applied, so they can only be sampled, which is not conducive to the immediate detection of large-scale cell samples, and long-term use of some biochemical reagents (such as trypan blue, 3H radioactive isotopes, etc.) will cause damage to the health of the operator
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Embodiment 1
[0027] Example 1 Cell Viability Measurement Based on Delayed Luminescent Kinetics
[0028] The system used for the measurement of delayed luminescence of cells such as figure 1 As shown, the system includes a light source, a sample chamber, a photomultiplier tube, a multi-channel photon counter, and a timing controller.
[0029] The light source is connected to the sample chamber through an excitation fiber, and a shutter 1 is provided at the end of the excitation fiber to control the light entering the sample chamber, and a quartz lens is also provided between the shutter 1 and the sample chamber to focus the light entering the sample chamber.
[0030] The sample chamber is connected to the photomultiplier tube through a light-collecting fiber, and a shutter 2 is set at the end of the light-collecting fiber to control light entering the photomultiplier tube, and the photomultiplier tube is electrically connected to a multi-channel photon counter.
[0031] The timing control...
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The invention discloses a method for detecting cell viability based on delayed luminescence kinetics. A specific mathematical model is used to fit the measured delayed luminescence decay curve of cells to obtain the values of various parameters. Among them, the parameter N s and N 0 The ratio of is the cell viability, the larger the value, the higher the cell viability, and the smaller the value, the lower the cell viability. Using this method to detect cell viability does not require exogenous markers and will not cause damage to cells or operators. It is safe and simple, and is suitable for instant detection of large-scale cell samples. In addition, it can display different viability states of cells in more detail. The various parameters below are for reference in later research.
Description
technical field [0001] The invention relates to the technical field of cell viability detection, in particular to a cell viability detection method based on delayed luminescence kinetics. Background technique [0002] Cell viability is an important indicator of cell biology. At present, biomedicine is mainly based on morphological and biochemical methods to detect cell viability. Morphological methods are convenient, direct, and highly visible, but generally they can only perform qualitative analysis, and it is difficult to provide quantitative detection information, and the accuracy of the analysis results depends on the experience of the experimenter, and there is a lot of interference from human factors. Biochemical methods are sensitive, accurate, and specific, and quantitative detection results can be provided by using flow cytometers, microplate readers, fluorescence analyzers, and spectrophotometers. However, these methods are often invasive, and the biochemical rea...
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IPC IPC(8): G01N21/63G16B5/00
CPCG01N21/63G16B5/00
Inventor 白华韩之波陈微朱涛刘杰
Owner TIANJIN POLYTECHNIC UNIV