Recombinant lactococcus lactis used for generating lycopene, and application of recombinant lactococcus lactis

Abstract

The invention discloses a recombinant lactococcus lactis bacterial strain used for generating lycopene, and a construction method and application of the recombinant lactococcus lacti. The recombinant lactococcus lactis bacterial strain is constructed in a way that parts of nucleotide sequences of two competition approaches, i.e., a lactic dehydrogenase gene and an [alpha]-acetolactic acid synthetase gene, of a lycopene synthesis precursor are eliminated, and then, plasmids are used to introduce a geranyl pyrophosphate synthetase gene (crtE), a phytoene synthetase gene (crtB) and a phytoene dehydrogenase gene (crtI) of a Pantoea ananatis source. Experiments prove that after the recombinant lactococcus lactis disclosed by the invention is subjected to shaking culture, the lycopene can be generated, and the experiments also predict that the recombinant lactococcus lactis has wide application potential in the development field of health care products and biological medicines.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a recombinant Lactococcus lactis producing lycopene and its construction method and application. Background technique

[0002] For centuries, Lactococcus lactis has been used in the fermentation of cheese, yogurt, sauerkraut and other foods, and is a safe microorganism recognized by the US Food and Drug Administration. Lactococcus lactis also has some probiotic properties, such as immunomodulatory properties, survivability through the gastrointestinal tract (GIT), but no intestinal colonization, etc., which make Lactococcus lactis a preferred delivery vehicle for biotherapeutic drugs. In 2000, Science magazine reported the use of recombinant Lactococcus lactis to secrete interleukin 10 to treat colitis in mice. In the following 20 years, Lactococcus lactis was used as a carrier for the delivery of nearly 60 therapeutic drugs, targeting diseases including enteritis, Flu, ...

Images