RNA extraction kit suitable for normal-temperature transportation, and extraction method

Abstract

The invention relates to an RNA extraction kit suitable for normal-temperature transportation. The kit comprises a lysis binding solution, and the lysis binding solution contains guanidine isothiocyanate with the concentration of 1 to 5 M, sodium thiocyanate with the concentration of 0.2 to 3 M, trihydroxymethyl aminomethane with the concentration of 0.01 to 0.1 M, tetrasodium iminodisuccinate with the concentration of 0.001 to 0.05 M, sodium ascorbate with the mass fraction of 0.05-1%, betaine with the mass fraction of 0.1-2%, Tween 20 with the mass fraction of 1-6%, sodium acetate with the concentration of 0.05 to 0.5 M, and isopropanol withthe mass fraction of 25-55%. The lysis binding solution does not contain toxic organic solvents such as phenol and does not contain reagents such as protease K needing cold-chain transportation, so that the RNA extraction kit can be transported under the condition of normal temperature. The lysis binding solution has the effects of lysis of cells / viruses and promotion of combination of nucleic acid and magnetic beads, can complete lysis and combination in one step, and is more suitable for an automatic nucleic acid extractor. The two protein denaturants are combined for use, and compared with single use of one protein denaturant, the capacity of removing impurities such as protein with complex chemical properties can be enhanced.

Description

technical field [0001] The invention relates to the technical field of nucleic acid processing, in particular to an RNA extraction kit and extraction method suitable for normal temperature transportation. Background technique [0002] With the development of detection technology, pathogenic nucleic acid detection has become the mainstream detection method for the diagnosis of respiratory pathogens due to its high sensitivity and high specificity detection performance. By detecting the nucleic acid of pathogens in respiratory specimens such as nasopharyngeal swabs, airway extracts or sputum, it can accurately and quickly detect and distinguish common respiratory system infections from malignant pathogenic infections, and make rapid diagnosis of pathogenic infections, and can quickly Distinguish the pathogen type and subtype / strain, and provide basis and guidance for subsequent targeted prevention and treatment measures. [0003] Nucleic acid extraction is the first step in p...

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned shortcomings and deficiencies of the prior art, the present invention provides an RNA extraction kit suitable for transportation at room temperature, which solves the problem that proteinase K-based RNA extraction kits cannot be transported at room temperature, and the RNA extraction kit of the present invention The kit does not contain toxic organic solvents and does not require heat treatment during the extraction of sample RNA. It uses one-step processing to complete lysis and binding at the same time. It is not necessary to separate lysis and binding. It is more suitable for automatic nucleic acid extraction instruments. The operation process is simple and fast, and it is also applicable Since the reagents are pre-packed into deep-well plates, the automated operation is easier and faster

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