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Single-frame differential phase contrast quantitative phase imaging method based on color multiplexing illumination

An imaging method and frame difference technology, which is applied in image enhancement, image analysis, image data processing, etc., can solve the problems of missing frequency information and slow imaging speed, and achieve quality improvement, fast imaging speed, and high stability quantitative phase imaging Effect

Active Publication Date: 2021-04-16
NANJING UNIV OF SCI & TECH
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Problems solved by technology

[0004] The purpose of the present invention is to provide a single-frame differential phase contrast quantitative phase imaging method based on color multiplexing illumination to solve the problems of slow imaging speed and serious lack of frequency information in differential phase contrast quantitative phase imaging, and realize real-time dynamic high resolution , High stability differential phase contrast quantitative phase imaging

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  • Single-frame differential phase contrast quantitative phase imaging method based on color multiplexing illumination
  • Single-frame differential phase contrast quantitative phase imaging method based on color multiplexing illumination
  • Single-frame differential phase contrast quantitative phase imaging method based on color multiplexing illumination

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Embodiment Construction

[0019] The experimental platform of the differential phase contrast quantitative phase microscopy imaging method based on color multiplexing illumination can be built based on any commercial microscope system, only need to add a light source modulation module color LCD display lighting or programmable color LED in the optical path array. Taking the differential phase contrast imaging system based on LCD display illumination as an example, the schematic diagram of the microscope system is as follows: figure 2 As shown in (a), it includes a color multiplexing light source module (mercury lamp, LCD, and condenser), sample, microscope objective lens (achromatic objective lens), tube lens, and color camera. The color multiplexing light source module can adopt two structures. The first one is to use the light source, LCD display screen and condenser mirror that come with the microscope as the lighting module. The function of the LCD is to modulate the light source so that the light...

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Abstract

The invention discloses a single-frame differential phase contrast quantitative phase imaging method based on color multiplexing illumination, which adopts a color multiplexing illumination scheme to realize single-frame differential phase contrast quantitative phase imaging. The single-frame color multiplexing illumination schemeadopts three illumination wavelengths of red r, green g and blue b to irradiate a sample at the same time, the frequency information of the sample in multiple directions is converted into intensity information on different channels of a single color image, and the frequency information in all directions is obtained through channel separation. According to the color multiplexing illumination scheme, only one collected image is needed, the transfer response of the phase transfer function of single-frame differential phase contrast imaging in the whole frequency range is enhanced, high-contrast, high-resolution and high-stability real-time dynamic quantitative phase imaging is achieved, an alternate illumination strategy is given, and completely isotropic imaging resolution is achieved at the camera acquisition limit speed.

Description

technical field [0001] The invention belongs to optical microscopic imaging and quantitative phase imaging technology, in particular to a single-frame differential phase contrast quantitative phase imaging method based on color multiplexing illumination. Background technique [0002] In the fields of biology, biomedicine and pathology research, the quantitative analysis of the three-dimensional structure of cells at the cellular and subcellular level is very necessary. However, biological cells exhibit low contrast under conventional brightfield microscopy due to their weak absorption. In order to make cells visible under a microscope, existing methods often use dyeing or fluorescent labeling methods to improve the contrast of cells under a microscope. However, this invasive approach often has an effect on cell viability, possibly damaging or even killing the cell. Label-free imaging technologies solve this problem, such as phase-contrast imaging (PC) and differential inte...

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Application Information

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IPC IPC(8): G02B21/36G02B21/06G06T5/00
CPCG06T5/10G06T2207/10101G06T2207/20056G02B21/367G02B21/14G06T5/90G06T5/50
Inventor 陈钱范瑶左超孙佳嵩潘向鹏冯世杰张玉珍顾国华李加基张佳琳
Owner NANJING UNIV OF SCI & TECH
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