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False positive sample elimination method and device, and electronic equipment

A false positive, sample technology, applied in instrumentation, biostatistics, data visualization, etc., can solve the problem of low accuracy in eliminating false positive samples

Pending Publication Date: 2021-04-16
HANGZHOU BIOER TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method, device and electronic equipment for eliminating false positive samples, so as to alleviate the low technical problem of eliminating false positive samples at present

Method used

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  • False positive sample elimination method and device, and electronic equipment
  • False positive sample elimination method and device, and electronic equipment
  • False positive sample elimination method and device, and electronic equipment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045]figure 1 It is a schematic flowchart of a method for eliminating false positive samples provided in the embodiment of the present application. like figure 1 As shown, the method includes:

[0046] Step S110, acquiring the amplification data of the unknown sample.

[0047] In step S120, a significantly amplified sample is determined from the amplification data of the unknown sample by a clustering method.

[0048] Step S130, calculating the standard deviation of the multiple cycle signals of the obviously amplified sample, the maximum amplification height of the obviously amplified sample, and the Ct-max value corresponding to the maximum amplification height.

[0049] Step S140, based on the standard deviation, the maximum amplification height, and the Ct-max value, exclude false positive samples from unknown samples.

[0050] From the amplification data of unknown samples by the clustering method, it is possible to determine the obviously amplified samples, and then ...

Embodiment 2

[0080] Figure 5 A schematic structural diagram of a device for eliminating false positive samples provided in the embodiment of the present application, as shown in Figure 5 As shown, the exclusion device 500 of false positive samples comprises:

[0081] Obtaining module 501, for obtaining the amplification data of unknown sample;

[0082] A determination module 502, configured to determine an obviously amplified sample from the amplification data of an unknown sample by a clustering method;

[0083] Calculation module 503, used to calculate the standard deviation of multiple cycle signals of obviously amplified samples, the maximum amplification height of obviously amplified samples, and the Ct-max value corresponding to the maximum amplification height;

[0084] The exclusion module 504 is configured to exclude false positive samples in unknown samples based on standard deviation, maximum amplification height and Ct-max value.

Embodiment 3

[0086] An electronic device provided by an embodiment of the present application, such as Image 6 As shown, the electronic device 600 includes a memory 601 and a processor 602, the memory stores a computer program that can run on the processor, and when the processor executes the computer program, the method provided by the above-mentioned embodiments is implemented. step.

[0087] see Image 6 , the electronic device further includes: a bus 603 and a communication interface 604, the processor 602, the communication interface 604 and the memory 601 are connected through the bus 603; the processor 602 is used to execute executable modules stored in the memory 601, such as computer programs.

[0088] Wherein, the memory 601 may include a high-speed random access memory (Random Access Memory, RAM for short), and may also include a non-volatile memory (non-volatile memory), such as at least one disk memory. The communication connection between the system network element and at ...

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Abstract

The invention provides a false positive sample elimination method and device, and electronic equipment, and relates to the technical field of data detection. The method comprises the following steps: obtaining the amplification data of an unknown sample, determining an obviously amplified sample from the amplification data of the unknown sample through a clustering method, calculating the standard deviation of the plurality of circulating signals of the obviously amplified sample, the maximum amplification height of the obviously amplified sample and the Ct-max value corresponding to the maximum amplification height, and eliminating the false positive sample in the unknown sample on the basis of the standard deviation, the maximum amplification height and the Ct-max value. The technical problem of relatively low accuracy of eliminating the false positive sample at present is alleviated.

Description

technical field [0001] The present application relates to the technical field of data detection, in particular to a method, device and electronic equipment for eliminating false positive samples. Background technique [0002] At present, the standard nucleic acid quantification method for diagnosing infectious diseases mostly uses fluorescent real-time quantitative PCR, which can quantify the initial value of the sample template, and is often used in gene analysis and expression, genetically modified food detection and cancer detection. [0003] However, the current actual PCR amplification curves are relatively diverse. Even in the same amplification experiment, the amplification curves of each well position are also very different. Moreover, the existing methods cannot eliminate the inconspicuous amplification more accurately. The analysis results of curve samples are different. At present, the accuracy of excluding false positive samples is low, and it is difficult to ins...

Claims

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Application Information

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IPC IPC(8): G16B45/00G16B40/30G16B40/10
Inventor 李冬杨智贺贤汉
Owner HANGZHOU BIOER TECH CO LTD