Application of lncrna XR_595534.2 in the preparation of drugs for the treatment or prevention of chronic pain

A drug and trigeminal neuralgia technology, applied in the field of biotechnology and medicine, can solve the problems of prone to gastric bleeding, poor curative effect of chronic neuropathic pain, dependence and addiction, and achieve the effect of alleviating pain behavioral responses

Active Publication Date: 2022-01-25
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former is only effective for mild to moderate pain, and serious side effects such as gastric bleeding are prone to occur after medication; the latter has a strong analgesic effect, but has poor curative effect on chronic neuropathic pain, and is accompanied by respiratory depression and constipation, especially after long-term use. Tolerance to its analgesic effects, which can lead to dependence and addiction

Method used

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  • Application of lncrna XR_595534.2 in the preparation of drugs for the treatment or prevention of chronic pain
  • Application of lncrna XR_595534.2 in the preparation of drugs for the treatment or prevention of chronic pain
  • Application of lncrna XR_595534.2 in the preparation of drugs for the treatment or prevention of chronic pain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: High-throughput sequencing results show lncRNAs differentially expressed in TG tissues of CCI-ION model rats

[0027] (1) Infraorbital nerve chronic compression injury (CCI-ION) model

[0028] Adult male healthy Sprague-Dawley rats weighing 180–250 g were provided by the Experimental Animal Center of Soochow University. Sanitary approval document number of the Animal Center: SYXK (Su) 2007-0035. Before the experiment, the animals were allowed to adapt to the feeding environment for three days and underwent adaptive stimulation training. The model was established using routine laboratory practices: the rats were anesthetized by intraperitoneal injection of 4% chloral hydrate at a dose of 1 ml / 100 g, and the rats were fixed on the operating table in a supine position. The first molar level was incised with a sterile razor blade, and the surrounding tissue was carefully separated using a bluntly bent glass rod until the infraorbital nerve was exposed. The two...

Embodiment 2

[0034] Example 2: The expression of lncRNA XR_595534.2 in TG tissue of CCI-ION model rats was significantly increased by fluorescence quantitative PCR

[0035] (1) establishment of infraorbital nerve chronic compression injury (CCI-ION) model (same as embodiment 1)

[0036] (2) Real-time fluorescent quantitative PCR

[0037] RNA extraction: Rat TG was extracted using sterilized equipment, placed in a 1.5 ml sterilized centrifuge tube, 1 ml Trizol was added, the tissue was broken into a homogenate, and placed on ice for 30 minutes. Add 100 microliters of chloroform, and centrifuge in a centrifuge at 4 degrees and 12000 rpm for 20 minutes. Aspirate the upper aqueous phase into a new centrifuge tube, add an equal volume of isopropanol and place it in the refrigerator at -20°C for 20 minutes. Centrifuge at 12,000 rpm for 15 minutes at 4°C in a centrifuge, discard the supernatant, and add 75% ethanol to wash the precipitate. Centrifuge at 4°C at 7500 rpm for 5 minutes, discard the...

Embodiment 3

[0040] Example 3: siRNA interference sequence can effectively reduce the expression of lncRNA XR_595534.2 in TG of CCI-ION model rats

[0041] (1) RNA extraction (same as Example 2).

[0042] (2) Real-time fluorescent quantitative PCR (same as Example 2).

[0043] (3) Stereotaxic injection of siRNA interference sequence into rat TG: After the rat was anesthetized, it was fixed on the brain stereotaxic apparatus, and the scalp was incised with a scalpel. 2 o 2 The tissue is corroded, exposing the coronal and sagittal sutures. Use the point of intersection of the two, namely bregma, as the origin: bregma is 3 mm backward, 3 mm to the left of the midline, and 11.7 mm below the surface of the skull. Inject lncRNA XR_595534.2 siRNA (lncRNA-siRNA) and control siRNA (NC-siRNA) (purchased from Gemma Gene), the final injection concentration is 50 micromole / liter, and each rat is injected with 3 microliters, and the injection is completed in 5 minutes. Leave the needle for 10 minute...

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Abstract

The invention discloses the application of lncRNA XR_595534.2 in the preparation of medicines for treating or preventing chronic pain. The present invention takes trigeminal neuralgia induced by chronic compression injury of infraorbital nerve in rats as a pain model, screens out a specific and differentially highly expressed long-chain non-coding RNA gene lncRNA XR_595534.2 in the model, and provides a therapeutic lncRNA XR_595534.2 is an interfering RNA for diseases targeted. The present invention discovers for the first time the lncRNA XR_595534.2 that is specifically and differentially expressed in pain models, and localized injection of interfering RNA can significantly reduce its expression and relieve pain behavioral responses, which can be used to prepare lncRNA‑XR595534.2 as the target Drugs for the prevention or treatment of trigeminal neuralgia, neuropathic pain, migraine and cancer pain.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to the application of lncRNA XR_595534.2 in the preparation of medicines for treating or preventing chronic pain. Background technique [0002] Chronic pain (such as trigeminal neuralgia, migraine, neuropathic pain, cancer pain, etc.) is a chronic disease that seriously endangers human physical and mental health and quality of life. Its characteristics make patients suffer for a long time, and can induce malignant emotions or mental abnormalities such as anxiety, depression and fear, and even cause suicidal tendencies in individuals, seriously endangering the life and quality of life of patients. Among the many preparations that can relieve pain, according to the pharmacological mechanism, they can be mainly divided into two categories: non-steroidal anti-inflammatory analgesics and opioid receptor agonists. The former is only effective for mild to moderate pain, and seri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/713A61K45/00A61P25/02A61P25/06C12N15/113
CPCA61K31/713A61K45/00A61P25/02A61P25/06C12N15/113C12N2310/14A61P25/04C12N2310/113C12N2330/10C12N15/11
Inventor 陶金齐任飞张园孙玉芳汤顺蒋星红
Owner SUZHOU UNIV
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