Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Keratin BD-6, preparation method, pharmaceutical composition and application thereof

A technology of BD-6 and keratin, applied in the field of keratin BD-6, its preparation method and its pharmaceutical composition and application, to achieve the effects of prolonging the incubation period, analgesic effect and strong effect

Pending Publication Date: 2021-04-30
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Using the protein expression system to prepare the target keratin, and then study its structure and function, no other literature reports, novel and creative

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Keratin BD-6, preparation method, pharmaceutical composition and application thereof
  • Keratin BD-6, preparation method, pharmaceutical composition and application thereof
  • Keratin BD-6, preparation method, pharmaceutical composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0115] Example 1 shake flask fermentation preparation protein BD-6 crude solution A (TB medium)

[0116] Synthesize the nucleotide sequence shown in SEQ ID No.2, and transfer it into the pET-28a (+) vector; sequencing confirms that the expression vector containing the correct sequence is obtained; the expression vector is transfected into BL21 (DE3) cells, The expression-competent host cells containing the target nucleotide sequence are obtained. Add it to LB medium, culture in a shaker at 37° C. and 220 rpm for 1 hour to obtain a recombinant strain.

[0117] The recombinant strain was dipped and streaked on the LBA plate containing Kanamycin, and the plate was placed upside down in a constant temperature incubator at 37°C for overnight cultivation for 16 hours.

[0118] Prepare 400ml of TB culture medium, divide into 2 bottles, each bottle is 200ml. Add Kanamycin (final concentration 50 μg / ml) to each bottle (200ml) of TB medium, take a single colony on the plate and add it...

Embodiment 2

[0124] Example 2 shake flask fermentation preparation protein BD-6 crude solution B (other medium)

[0125] Synthesized and sequenced in Example 1 to obtain an expression vector containing the sequence shown in SEQ ID No.2; the expression vector was transfected into BL21 (DE3) cells to obtain expression-competent host cells containing the target nucleotide sequence.

[0126] Prepare 20 ml of LB medium, take 800 μl, add 50 μl of host cells containing the target coding sequence, and culture in a shaker at 37° C. and 220 rpm for 1 hour.

[0127] Dip the above bacterial solution and streak it on the LBA plate containing Kanamycin, and place the plate upside down in a constant temperature incubator at 37°C for overnight cultivation for 16 hours.

[0128] Take 10ml of LB medium, add Kanamycin (final concentration 50 μg / ml), take a single colony on the plate and add it to LB medium, in a shaker, under the conditions of 37°C and 220rpm overnight amplification culture for 15 hours, to ...

Embodiment 3

[0134] Example 3 fermenter preparation protein BD-6 crude solution C

[0135] Synthesized and sequenced in Example 1 to obtain an expression vector containing the sequence shown in SEQ ID No.2; the expression vector was transfected into BL21 (DE3) cells to obtain expression-competent host cells containing the target nucleotide sequence. Add it to LB medium, culture in a shaker at 37° C. and 220 rpm for 1 hour to obtain a recombinant strain.

[0136] On the LBA plate containing Kanamycin, add 100 μl of the recombinant strain, spread it with a spreader until it dries evenly, and place the plate upside down in a constant temperature incubator at 37°C for overnight culture. Take three single colonies and streak them on a plate containing Kanamycin, culture the plate overnight, and after three batches of shake flask fermentation and expression verification confirm that it is correct, preserve the strain with 15% glycerol, and pack it into 0.8ml each to obtain Working cell bank, fr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to keratin BD-6, a nucleic acid molecule encoding the keratin BD-6, an expression vector containing the nucleic acid molecule, a host cell containing the expression vector or a genome integrating the nucleic acid molecule, a preparation method of the keratin BD-6, and a pharmaceutical composition containing the keratin BD-6. The invention further discloses an application of the keratin BD-6, the nucleic acid molecule, the expression vector, a host cell or the pharmaceutical composition in preparation of antipyretic, analgesic, antitussive, expectorant, anticonvulsant, antiepileptic, antihypertensive, anti-inflammatory and antiviral drugs.

Description

technical field [0001] The present invention relates to a keratin BD-6, a nucleic acid molecule encoding keratin BD-6, an expression vector containing the nucleic acid molecule, a host cell containing the expression vector or a genome integrating the nucleic acid molecule, and keratin BD-6 The preparation method, the pharmaceutical composition containing this keratin, and this keratin and said pharmaceutical composition are used in the preparation of antipyretic and analgesic, antitussive and expectorant, anticonvulsant, antiepileptic, hypotensive, anti-inflammatory, anti-inflammatory Applications in viral medicine. Background technique [0002] Keratin is a kind of protein, which widely exists in the epidermis of humans and animals, and is the main component of hair, feathers, hooves, shells, claws, horns, etc. It is an extremely important structural protein of connective tissue and plays a role in protecting the body . [0003] Keratin exists widely in living organisms a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12A61K38/17A61P29/00A61P25/00A61P25/08A61P11/14A61P11/10A61P9/12A61P31/12
CPCC07K14/4741A61P29/00A61P25/00A61P11/14A61P11/10A61P25/08A61P9/12A61P31/12A61K38/00A61P11/00C12N15/70A61P31/00B01D61/147C07K1/16C12N15/63
Inventor 庾石山王晓良史国茹刘云宝李勇王玲李江冯楠李密
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com