Construction and application of an animal model of chronic drug toxicity retinal photoreceptor cell injury

A technology of photoreceptor cells and drug toxicity, applied in pharmaceutical formulations, compound screening/testing, preparations for in vivo experiments, etc., to achieve the effect of effective understanding

Active Publication Date: 2022-07-19
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently no animal models of chronic retinal damage due to drug toxicity

Method used

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  • Construction and application of an animal model of chronic drug toxicity retinal photoreceptor cell injury
  • Construction and application of an animal model of chronic drug toxicity retinal photoreceptor cell injury
  • Construction and application of an animal model of chronic drug toxicity retinal photoreceptor cell injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0035] Example: MMS-induced establishment of an animal model of chronic retinal photoreceptor cell damage

[0036] 1. Selection of experimental animals:

[0037] Healthy SPF grade C57BL / 6J male mice (8-10 weeks old, purchased from Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd.), rearing conditions: temperature 20-25°C, alternating light and dark for 12 / 12h, and unlimited water. The use of experimental animals follows the relevant regulations of ARVO and meets the relevant requirements of my country's "Regulations on the Administration of Laboratory Animals".

[0038] 2. MMS solution preparation and drug dosage

[0039] To establish an animal model of chronic drug toxicity retinal photoreceptor cell damage, based on previous practical research, two series of seven treatment doses of MMS were selected: 75mg / (kg·body weight), 37.5mg / (kg·body weight), 18.75mg / ( kg body weight), 9.375mg / (kg body weight) and 60mg / (kg body weight), 50mg / (kg body weight), 40mg / (kg body...

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PUM

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Abstract

The invention relates to the construction and application of an animal model of chronic drug toxicity retinal photoreceptor cell damage, and aims to solve the technical problem that there is no relevant animal model in the prior art. The present invention mainly constructs an animal model of chronic drug toxicity retinal photoreceptor cell damage by injecting methyl methanesulfonate into the tail vein of mice at a dosage of 40-60 / (kg·body weight). The invention establishes an animal model of chronic drug retinal photoreceptor cell injury for the first time, and can provide a reliable and stable animal model for studying the molecular regulation effect of epigenetic modification on the transdifferentiation and regeneration of endogenous stem cells in the process of retinal injury, pathogenesis and intervention therapy It also lays a material and technical foundation for people to more effectively understand the mechanism and development of retinal drug toxicity diseases and to study intervention and treatment measures.

Description

technical field [0001] The invention relates to the technical field of experimental animal models, in particular to the construction and application of an animal model of chronic drug toxicity retinal photoreceptor cell damage. Background technique [0002] Vision is one of the important senses for humans and animals to obtain information from the outside world. The retina is an important organ of visual perception, composed of tertiary neurons, that is, photoreceptor cells sense light signals, convert the light signals into electrical signals through synaptic connections, and transmit them to bipolar cells, and then the optic nerves formed by the axons of ganglion cells go to the optic nerve. The visual center of the cerebral cortex transmits and constitutes vision. The retina is composed of 5 main cell components and 2 types of glial cell bodies and axons to form a 10-layer structure of the retina. [0003] In clinical medicine, there are many drugs with retinal drug tox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/027A61K49/0008A01K2227/105A01K2267/03
Inventor 彭广华纪艳丽
Owner ZHENGZHOU UNIV
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