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Method for distinguishing donkey-hide gelatin from other animal-derived gelatin based on GC-IMS technology and application

A GC-IMS, animal source technology, applied in the direction of material separation, analysis materials, instruments, etc., can solve the problems of complex pretreatment process, complex detection method, time-consuming and other problems, and achieve fast detection speed, simple detection method and high sensitivity Effect

Pending Publication Date: 2021-05-14
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the inventors found that the detection method is complicated and time-consuming to extract the characteristic peptides and compare and verify the sequence and structure.
In particular, both PCR and specific peptide identification techniques need to use specific methods to pre-treat samples, the pre-treatment process is very complicated, and corresponding standard peptides are also required

Method used

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  • Method for distinguishing donkey-hide gelatin from other animal-derived gelatin based on GC-IMS technology and application
  • Method for distinguishing donkey-hide gelatin from other animal-derived gelatin based on GC-IMS technology and application
  • Method for distinguishing donkey-hide gelatin from other animal-derived gelatin based on GC-IMS technology and application

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Embodiment 1

[0052] Sample processing: samples DHG (donkey hide glue), CHG (cowhide glue), HHG (horse hide glue) and PHG (pig hide glue) were crushed, weighed 2g samples respectively, placed in 20mL headspace bottles, and incubated at 60°C 500 mL was injected after 15 minutes.

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Abstract

The invention provides a method for distinguishing donkey-hide gelatin from other animal-derived gelatin based on a GC-IMS technology and application. The method comprises the step of detecting a crushed sample to be detected by adopting the GC-IMS technology. Compared with the prior art, the method has the advantages that the sample cannot be pretreated, extraction, concentration and other treatments are not needed, the sample can be directly subjected to sample injection detection after being directly crushed, the detection time is greatly saved, and the accuracy is high.

Description

technical field [0001] The invention relates to the technical field of authenticity identification of donkey-hide gelatin, in particular to a method and application for distinguishing donkey-hide gelatin from other animal-derived gums based on GC-IMS technology. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] For a long time, the key material of donkey-hide gelatin is donkey skin, which comes from equine donkeys. Ejiao is one of the few traditional Chinese medicines with clear curative effects. Donkey-hide gelatin contains gelatin, protein, lysine, histidine, arginine, threonine and trace elements and other active ingredients, which are resistant to hypoxia, cold, fatigu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72
Inventor 耿越马宁
Owner SHANDONG NORMAL UNIV