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Culture solution for colorectal cancer organoid culture and preparation method thereof

A technology of colorectal cancer and culture fluid, applied in the field of biomedicine, can solve the problems of lack of research models, and the survival rate of patients with treatment efficiency has not been significantly improved.

Active Publication Date: 2021-05-18
杭州艾名医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The underlying mechanisms of colorectal cancer pathobiology are currently poorly understood, and treatment efficiency and patient survival have not significantly improved, mainly due to the lack of suitable research models

Method used

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  • Culture solution for colorectal cancer organoid culture and preparation method thereof
  • Culture solution for colorectal cancer organoid culture and preparation method thereof
  • Culture solution for colorectal cancer organoid culture and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] The culture solution used for the culture of colorectal cancer organoids in this example is made of the following raw materials per 50 mL of culture solution: icabet sodium, with a final concentration of 1 μg / mL; vitamin P4, with a final concentration of 100 μM; human recombinant protein R-Spondin 1, the final concentration is 30ng / mL; noggin, the final concentration is 100ng / mL; human recombinant protein rhEGF, the final concentration is 8ng / mL; 4-hydroxyethylpiperazineethanesulfonic acid, the final concentration is 1mM; L-alanyl-L-glutamine, the final concentration is 1×; blue chain double antibody, the final concentration is 1×; N2 additive, the final concentration is 1×; B27 additive, the final concentration is 1×; N-acetyl- L-cysteine, the final concentration is 0.09mM; Alk inhibitor A83-01, the final concentration is 0.5μM; 4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4 -pyridyl)-1H-imidazole with a final concentration of 0.3 μM; gastrin with a final concentration of...

Embodiment 2

[0074] The culture medium used for culturing colorectal cancer organoids in this example is made of the following raw materials per 50 mL of culture medium: icabet sodium, with a final concentration of 50 μg / mL; vitamin P4, with a final concentration of 50 μM; human recombinant protein R-Spondin 1, the final concentration is 40ng / mL; noggin, the final concentration is 90ng / mL; human recombinant protein rhEGF, the final concentration is 9ng / mL; 4-hydroxyethylpiperazineethanesulfonic acid, the final concentration is 1mM; L-alanyl-L-glutamine, the final concentration is 1×; blue chain double antibody, the final concentration is 1×; N2 additive, the final concentration is 1×; B27 additive, the final concentration is 1×; N-acetyl- L-cysteine, the final concentration is 0.11mM; Alk inhibitor A83-01, the final concentration is 0.4μM; 4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4 -pyridyl)-1H-imidazole at a final concentration of 0.4 μM; gastrin at a final concentration of 10 nM; non-es...

Embodiment 3

[0078] The culture solution used for the culture of colorectal cancer organoids in this example is made of the following raw materials per 50 mL of culture solution: icabet sodium, with a final concentration of 100 μg / mL; vitamin P4, with a final concentration of 1 μM; human recombinant protein R-Spondin 1, the final concentration is 50ng / mL; noggin, the final concentration is 80ng / mL; human recombinant protein rhEGF, the final concentration is 10ng / mL; 4-hydroxyethylpiperazineethanesulfonic acid, the final concentration is 1mM; L-alanyl-L-glutamine, the final concentration is 1×; blue chain double antibody, the final concentration is 1×; N2 additive, the final concentration is 1×; B27 additive, the final concentration is 1×; N-acetyl- L-cysteine, the final concentration is 0.125mM; Alk inhibitor A83-01, the final concentration is 0.3μM; 4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4 -pyridyl)-1H-imidazole at a final concentration of 0.5 μM; gastrin at a final concentration of 10...

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Abstract

The invention discloses a culture solution for colorectal cancer organoid culture and a preparation method thereof, and belongs to the technical field of biological medicine. The culture solution is prepared from the following raw materials of ecabet sodium, vitamin P4, human recombinant protein R-Spond i n 1, noggin, human recombinant protein rhEGF, 4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid, L-alanyl-L-glutamine, penicillin-streptomycin solution, an N2 additive, a B27 additive, N-acetyl-L-cysteine, an Al k inhibitor A83-01, 4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)-1H-imidazole, gastrin, a non-essential amino acid solution, nicotinamide and an Advanced DMEM / F-12 culture medium. According to the culture solution disclosed by the invention, colorectal cancer tissue cells can keep high cell and tissue activity in the culture process, and the inherent pathological features of such cells can be better embodied.

Description

technical field [0001] The invention relates to a culture solution for culturing colorectal cancer organoids and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Colorectal cancer is one of the most common malignant tumors that seriously threaten human health. In my country, the incidence of colorectal cancer ranks third among all malignant tumors. The mortality rate of colorectal cancer ranks fifth in all malignant tumors. With the development of the economy, the improvement of living standards and the change of lifestyle, the incidence of colorectal cancer will continue to rise. [0003] Colorectal cancer is a complex disease, its occurrence and development is a dynamic process, involving the interaction of many signaling molecules, forming a complex molecular regulatory network, and also affected by external environmental factors, the etiology of colorectal cancer There are strong individual differences in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2500/38C12N2501/11C12N2500/30C12N2500/33C12N2501/998C12N2501/999
Inventor 游明亮王磊申重阳
Owner 杭州艾名医学科技有限公司