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Construction and application of chronic epilepsy rat model

A rat model, epilepsy technology, applied in the direction of medical preparations containing active ingredients, organic active ingredients, microbe determination/testing, etc., can solve the problem that the mechanism of epilepsy has no theoretical basis, etc.

Pending Publication Date: 2021-05-28
湖南省脑科医院
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  • Description
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  • Application Information

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Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides the construction and application of a rat model of chronic epilepsy, which solves the problem that the mechanism of LFS for epilepsy has no theoretical basis

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  • Construction and application of chronic epilepsy rat model
  • Construction and application of chronic epilepsy rat model
  • Construction and application of chronic epilepsy rat model

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Embodiment

[0026] like Figure 1-3 As shown, the embodiment of the present invention provides the construction and application of a chronic epilepsy rat model. In order to study the epilepsy treatment mechanism of low-frequency electrical stimulation (LFS), this research intends to select 6-8 weeks old male SD rats to construct chlorine Lithium-pilocarpine chronic epilepsy rat model, LFS treatment model rats, stimulate the hippocampus, monitor and record the EEG and behavior of the experimental rats, and use RT-PCR and Western blot to detect Rho in the hippocampal tissue fluid / ROCK signaling pathway related RhoA, ROCK-I and ROCK-II gene and protein expression changes, and retained hippocampal tissue for parallel pathological sections and immunohistochemistry, to explore whether LFS treatment of epilepsy is based on Rho / ROCK signaling pathway The main research contents are as follows: 1. Establishment and evaluation of lithium chloride-pilocarpine chronic epilepsy rat model; 2. LFS stimu...

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Abstract

The invention provides construction and application of a chronic epilepsy rat model, and relates to the technical field of epilepsy treatment research. According to construction and application of the chronic epilepsy rat model, in order to research an epilepsy treatment mechanism of low-frequency electrical stimulation (LFS), a male SD rat of 6-8 weeks old is selected, a lithium chloride-pilocarpine chronic epilepsy rat model is constructed, the model rat is treated through LFS, the hippocampus part is stimulated, and an electroencephalogram and behavioristics of the experimental rat are monitored and recorded; and gene level and protein level expression changes of RhoA, ROCK-I and ROCK-II related to Rhoo / ROCK signal passages in hippocampus tissue fluid are detected by applying RT-PCR and Westernblot methods, hippocampus tissues are reserved for parallel pathological section and immunohistochemistry, and whether LFS plays a role in treating epilepsy based on the Rhoo / ROCK signal passages or not is discussed. According to the invention, the theoretical basis is provided for preventing and treating epilepsy by discussing whether the LFS plays a role in improving epilepsy through the Rho / ROCK signal passages or not.

Description

technical field [0001] The invention relates to the technical field of epilepsy treatment research, in particular to the construction and application of a chronic epilepsy rat model. Background technique [0002] Epilepsy (epilepsy) is a serious chronic central nervous system disease, which is a temporary brain dysfunction caused by recurrent and highly synchronized abnormal discharge of brain neurons. The clinical manifestations are motor, sensory, consciousness, autonomic and mental It is one of the more common diseases of the nervous system, affecting more than 65 million people worldwide. The disability, death, and complications associated with epilepsy impose a heavy burden on patients and society. At present, little is known about the mechanism of epilepsy, which is not conducive to the prevention and treatment of epilepsy. Contents of the invention [0003] (1) Solved technical problems [0004] Aiming at the deficiencies in the prior art, the present invention p...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K31/4178A61K33/14C12Q1/6851G01N33/573G01N33/577A61N1/36
CPCA61K33/14A61K31/4178A01K67/027C12Q1/6851G01N33/577G01N33/573A61N1/36064A01K2267/0356C12Q2531/113C12Q2563/107C12Q2545/113C12Q2521/107
Inventor 卢军彭琼李振光刘坤朱勇匡卫平黄亚辉邓兰秋子尹佃敏周惠彧
Owner 湖南省脑科医院
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