Primer group for gram-positive bacterium drug-resistant gene high-throughput amplicon sequencing and application

A technology of Gram-positive bacteria and drug-resistant genes, applied in the direction of recombinant DNA technology, microbial measurement/testing, biochemical equipment and methods, etc., can solve the problems of difficult treatment of infectious diseases and limited treatment methods
CN112852938AActive Publication Date: 2021-05-28CHINA AGRI UNIV

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2021-05-28

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Abstract

The invention discloses a primer group for gram-positive bacterium drug-resistant gene high-throughput amplicon sequencing and application of the primer group. The invention provides a complete set of primers for gram-positive bacterium drug-resistant gene high-throughput amplicon sequencing. The complete set of primers provided by the invention consists of primers as shown in sequences 1-78. The invention discloses an amplicon sequencing detection analysis method for 39 clinical common drug-resistant genes. The method has the technical advantages of rapidness, high efficiency, high targeting, strong specificity and the like, and is suitable for epidemic and difference analysis of various drug-resistant genes in clinic and livestock breeding industry.
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Description

technical field

[0001] The invention belongs to the technical field of high-throughput sequencing of microbial genes, and in particular relates to a primer set for high-throughput amplicon sequencing of drug-resistant genes of Gram-positive bacteria and its application. Background technique

[0002] Metagenomics, also known as microbial environmental genomics, metagenomics. By directly extracting the DNA of all microorganisms from environmental samples, constructing a metagenomic library, using metagenomic sequencing to perform high-throughput sequencing of microorganisms, analyzing the composition, diversity and corresponding functions of all microorganisms in environmental samples, and then exploring the relationship between microorganisms and The relationship between environment, microbe and host. Metagenomics, developed on the basis of microbiomics, does not rely on the isolation and culture of microorganisms, and can be used to study natural products and natural produc...

Claims

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