Detection system for urinary system infection pathogens and kit and application thereof

Abstract

The invention relates to a urinary system infection pathogen detection system and a kit and application thereof, the detection system comprises 20 pairs of primers, including 18 pairs of detection primers, one pair of human DNA internal reference primers and one pair of system quality control internal reference primers. According to the detection system for the drug-resistant gene of the urinary system infection pathogen as well as the kit and the application of the detection system, conventional detection is not needed, and synchronous identification and quantitative analysis of various pathogens can be directly carried out on a urine sample in the same reaction system. The system has the advantages of strong specificity, high sensitivity, good repeatability, rapidness and accuracy, provides comprehensive, accurate and low-cost etiological diagnosis for clinic in the first time, and provides important reference for individualized medication and precise medical treatment.

Description

technical field [0001] The invention relates to a multiple gene detection product and a detection system used in the product, belonging to the field of biotechnology. Background technique [0002] Urinary system infection, also known as urinary tract infection (Urinary Tract Infections, UTI), is one of the most common infectious diseases clinically. A variety of pathogens can invade the urinary system and grow and reproduce in large numbers, causing clinical symptoms such as frequent urination, urgency, dysuria, dysuria, and waist and leg pain. In severe cases, severe diseases such as sepsis and acute and chronic renal dysfunction may develop; However, urogenous sepsis and septic shock progress very rapidly, and the fatality rate is as high as 28.3% to 41.1%. UTI accounts for about 40% of nosocomial infections in foreign countries, ranking first; while the incidence of UTI in my country is as high as 20.8% to 31.7%, second only to respiratory tract infections, and more than...

AI Technical Summary

Problems solved by technology

However, there are many deficiencies in culture identification: ①Long time-consuming: ordinary bacterial culture incubation takes 16 to 24 hours, and it takes even longer for pathogens with slow growth and complex nutritional requirements; biochemical identification and drug sensitivity test are required after the colony grows, It takes at least 3 working days; ②Incomplete coverage and low positive detection rate: Because ordinary culture is mostly aimed at a single pathogen, and UTI also has difficult-to-cultivate but extremely important pathogenic bacteria such as Mycobacterium tuberculosis and Mycoplasma, which often lead to false negative

However, the current conventional detection methods have the disadvantages of low detection rate, long time-consuming, especially the inability to accurately identify multiple pathogens at the same time, and cannot provide timely, comprehensive and accurate pathogen diagnosis basis for clinical practice, leading to the widespread use of broad-spectrum Empirical treatment with antibacterial drugs leads to low curative effect, failure to control the progress of urinary tract infection in time, high drug resistance rate of induced strains, and increased medical burden on patients, etc.

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