Preparation method of gel shell of electrophoresis gel

Abstract

The invention relates to a preparation method of a gel shell of electrophoresis gel, and is used for preventing leakage during preparation of the electrophoresis gel. The preparation method comprises the following steps: (a) providing the gel shell of the electrophoresis gel, which comprises a first flat plate, a second flat plate and a plurality of spacing components, wherein the first flat plate and the second flat plate respectively comprise an upper edge, a lower edge, a left edge, a right edge, an inner side surface and an outer side surface, when the inner side surfaces of the first flat plate and the second flat plate are correspondingly combined in parallel, a plurality of spacing components are arranged on the left side and the right side between the first flat plate and the second flat plate to form an electrophoresis colloid accommodating space; (b) a bonding step: bonding the first flat plate, the second flat plate and the plurality of spacing components; and (c) a cutting step: cutting an overlapped part of the lower sides of the first flat plate and the second flat plate so as to form a continuous planar structure on the lower edges of the first flat plate and the second flat plate.

Description

technical field [0001] The invention relates to a method for preparing a gel shell of an electrophoretic gel, in particular to a method for forming a continuous planar structure at the bottom of the gel shell through a cutting step, thereby avoiding leakage during the preparation of the electrophoretic gel. Background technique [0002] Electrophoresis is a method commonly used in the field of molecular biology to identify and separate biological macromolecules. The principle is to make use of the differences in molecular weight and charge value between different biomacromolecules to make these molecules propagate at different speeds in the medium under the influence of an electric field, and then separate these different biomacromolecules in the process. In order to facilitate the identification of molecular biology. [0003] The medium commonly used to separate biomacromolecules in electrophoresis is an electrophoretic gel, which is usually flat and has regularly spaced w...

AI Technical Summary

Problems solved by technology

However, the most important problem to overcome is that when the bottom of the shell of the electrophoretic gel cannot form a continuous planar structure, the liquid matrix from which the gel is made leaks even when sealed with tape

[0005] At present, there are some methods of making electrophoretic gel shells to avoid the leakage of the liquid matrix of the prepared gel. For example, Bio-Rad has established an integrally formed window pane structure at the bottom of the gel shell of the electrophoretic gel to avoid when the gel shell is assembled. The bottom is uneven; another example is that after the shell of the electrophoretic gel is assembled, the bottom is rolled with a hot roller to make it flat, but the risk of this design is the increase in cost and the lack of product yield

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