Visual tumor cell detection reagent and kit as well as preparation method and application thereof

Abstract

The invention discloses a visual tumor cell detection reagent and kit as well as a preparation method and application thereof. A tumor cell capturing material is prepared from a nano material, aggregation-induced emission molecules, a high-molecular compound with positive charge and a connecting body. The CTC capturing material has the universality (is capable of detecting cells from different tumor sources) and specificity, does not need a magnetic bead polyclonal antibody for surface modification, is low in cost and short in detection time, adopts a Turn-on type fluorescence mode, and avoids strong fluorescence background caused by an Always-on fluorescent dye, and false negative caused by cell loss and possibly occuring in the process of repeatedly washing off dye molecules which are not combined with the cells.

Description

technical field [0001] The invention belongs to the field of materials, and in particular relates to a visualized tumor cell detection reagent, kit, preparation method and application thereof. Background technique [0002] Malignant tumor is an important disease that endangers human life and health. Improving its early detection rate is one of the main methods to reduce tumor mortality. In related technologies, some routine detection methods for tumors, such as imaging methods, can only be detected when the tumor grows to a certain volume, but according to statistics, about 80% of the tumors are in the middle and late stages at this time. Pathological diagnosis is the premise and basis for the diagnosis and treatment of malignant tumors. Puncture or surgical biopsy is also a common method for diagnosing tumors. However, this invasive operation cannot be repeated frequently, and the difficulty of obtaining metastatic lesions also limits the application of biopsy. Multiple dy...

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Problems solved by technology

However, EpCAM is not a universal tumor marker for all CTCs and cannot detect CTCs of non-epithelial origin

There is also a negative enrichment method that uses CD45 immune-coated magnetic beads to adsorb leukocytes and remove leukocytes under the action of a magnetic field to enrich and precipitate circulating CTCs. However, the purity of this method is low, and the remaining leukocytes after enrichment can still interfere with subsequent analyze

The enrichment method based on physical characteristics, including microporous filtration method, captures CTCs based on the fact that their volume is larger than that of blood cells, but this method is not suitable for smaller CTCs

[0005] To sum up, the current capture methods for CTCs have significant limitations. For example, the detection technology based on specific tumor markers is only applicable to this type of tumor; the microfiltration method is only applicable to large cell tumors, etc. Therefore, , it is urgent to develop a novel tumor cell targeting method

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