Device for measuring light-activated fluorescence and its use

An excitation light and instrument technology, applied in the field of fluorescence instruments, can solve the problems of difficulty in miniaturization, low light, and inability to ensure accurate local distribution of fluorescence intensity, and achieve the effects of low-cost production and operation, miniaturization of structure, and reduced impact.

Inactive Publication Date: 2003-11-26
马蒂亚斯·劳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The measurement methods and instruments commonly used today suffer from the following disadvantages: the ratio of fluorescence to the light required to excite the fluorescence is too low, resulting in the need for separate devices, thus making the miniaturization required in many applications difficult
[0007] However, this method does not ensure a precise local distribution of detectable fluorescence intensity on the surface of the excited layer

Method used

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  • Device for measuring light-activated fluorescence and its use
  • Device for measuring light-activated fluorescence and its use
  • Device for measuring light-activated fluorescence and its use

Examples

Experimental program
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Embodiment Construction

[0067] A first exemplary embodiment of the apparatus of the present invention is shown in figure 1 middle.

[0068] In this figure, there is at least one light source 2 in the closed measuring head 1, which emits excitation light, which is transmitted to the fluorescent layer 11 by the optical fiber 3 through the filter 6. The filter 6 is preferably a replaceable bandpass type, and the optical fiber 3 reaches the fluorescent layer along the upper part 17 of the measuring head. Fluorescence emitted by the fluorescent layer 11 passes through a second optical fiber 15 via a replaceable wedge filter 7, which may be replaceable, to a detector 4 connected to an electronic evaluation system 9, where the intensity of the fluorescence can be measured.

[0069] Then the reflected light as a reference signal passes through the third optical fiber 16 , and may also pass through the replaceable filter 8 to reach the second detector 5 connected to the second electronic system 10 .

[0070...

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PUM

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Abstract

The invention relates to a device for measuring light-activated fluorescence of at least one coating that contains a fluorescent material, and its use for measuring fluid materials which cause fluorescence-quenching in at least one of the fluorescent coatings. To activate the fluorescence, at least one first light wave guide is directed onto at least one coating applied to a support and the fluorescent light is directed at a detector by means of at least one second light wave guide, in order to determine the intensity of the fluorescent light. The end faces of the different fluorescent light wave guides are then arranged, taking into account the numerical apertures of the different light wave guides and / or with reference to at least one coating containing a fluorescent material, in such a way that a local coordination of the measurable fluorescence intensity can be attained, and that the light source(s), light wave guides and the detector(s) are lodged in a measuring head.

Description

technical field [0001] The invention relates to an apparatus for measuring the fluorescence excited by light emitted by at least one layer containing a fluorescing substance; and to the use of the apparatus for measuring the effects of fluorescence in at least one fluorescing layer. Extinguished fluid substance. technical background [0002] The measurement methods and instruments commonly used today have the disadvantage that the ratio of fluorescence to the light required to excite the fluorescence is too low, resulting in the need for separate devices, making the miniaturization required in many applications difficult. [0003] Moreover, the existing methods for solving the problem cannot achieve a satisfactory separation effect between the excitation light and the fluorescence. [0004] For this reason, expensive and complex optical designs must be used, which requires many optical components, which increases the cost. In particular, problems such as miniaturization an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/85
CPCG01N2021/6484G01N2021/7786G01N2021/772G01N21/7703G01N21/8507G01N2021/6432
Inventor 马蒂亚斯·劳
Owner 马蒂亚斯·劳
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