Device for measuring light-activated fluorescence and its use

Abstract

The invention relates to a device for measuring light-activated fluorescence of at least one coating that contains a fluorescent material, and its use for measuring fluid materials which cause fluorescence-quenching in at least one of the fluorescent coatings. To activate the fluorescence, at least one first light wave guide is directed onto at least one coating applied to a support and the fluorescent light is directed at a detector by means of at least one second light wave guide, in order to determine the intensity of the fluorescent light. The end faces of the different fluorescent light wave guides are then arranged, taking into account the numerical apertures of the different light wave guides and / or with reference to at least one coating containing a fluorescent material, in such a way that a local coordination of the measurable fluorescence intensity can be attained, and that the light source(s), light wave guides and the detector(s) are lodged in a measuring head.

Description

technical field [0001] The invention relates to an apparatus for measuring the fluorescence excited by light emitted by at least one layer containing a fluorescing substance; and to the use of the apparatus for measuring the effects of fluorescence in at least one fluorescing layer. Extinguished fluid substance. technical background [0002] The measurement methods and instruments commonly used today have the disadvantage that the ratio of fluorescence to the light required to excite the fluorescence is too low, resulting in the need for separate devices, making the miniaturization required in many applications difficult. [0003] Moreover, the existing methods for solving the problem cannot achieve a satisfactory separation effect between the excitation light and the fluorescence. [0004] For this reason, expensive and complex optical designs must be used, which requires many optical components, which increases the cost. In particular, problems such as miniaturization an...

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Problems solved by technology

[0002] The measurement methods and instruments commonly used today suffer from the following disadvantages: the ratio of fluorescence to the light required to excite the fluorescence is too low, resulting in the need for separate devices, thus making the miniaturization required in many applications difficult

[0007] However, this method does not ensure a precise local distribution of detectable fluorescence intensity on the surface of the excited layer

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