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Lactobacillus rhamnosus preparation for enhancing immunity of human body and preparation method thereof

A technology of Lactobacillus rhamnosus and immunity, which is applied in the field of probiotics production, can solve the problems of inability to reflect the effect of metabolites of Lactobacillus rhamnosus, high bacterial stress mortality, and high bacterial mortality, so as to achieve enhanced The effects of human immunity, low mortality, and high bacterial activity

Pending Publication Date: 2021-06-11
盐城维康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This production method removes the metabolites produced by probiotics in the liquid fermentation process that are helpful for regulating human immunity, and the role of metabolites of Lactobacillus rhamnosus cannot be reflected in the product. Therefore, the probiotics produced by this method Probiotic products only use the effect of the probiotic itself but fail to utilize the effect of its metabolites; in addition, the stress mortality rate of the bacteria is high when dry, and due to the addition of an appropriate amount of dry protection agent or carrier in the wet bacteria, the The actual number of bacterial cells obtained after drying of liquid fermented products is also reduced
[0007] Therefore, the existing probiotic production process has defects such as low strain content, poor fermentation effect, high bacterial mortality during drying, and low bacterial strain viability in the finished product.

Method used

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  • Lactobacillus rhamnosus preparation for enhancing immunity of human body and preparation method thereof
  • Lactobacillus rhamnosus preparation for enhancing immunity of human body and preparation method thereof
  • Lactobacillus rhamnosus preparation for enhancing immunity of human body and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Isolation, strain characteristics and identification of embodiment 1 bacterial strain

[0034] 1.1 Sample collection

[0035] The samples of Lactobacillus rhamnosus were isolated and purified from the probiotic preparations sold by our company.

[0036] 1.2 Isolation of strains

[0037] Separation and purification are carried out in samples of probiotic preparations sold by our company. Take 4g of the probiotic preparation, put it into a sterile 50mL centrifuge tube, add 20mL of sterile normal saline, oscillate and mix well, place it in a water-proof incubator at 30°C, let it stand for overnight culture, draw 1mL of the sample solution, and take Bacterial normal saline was serially diluted 10 times to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 For each gradient, 100 μL of the bacterial suspension was spread on the MRS plate, and placed in an anaerobic incubator at 37°C for upside-down culture. After 48 hours, colonies were selected from the coated culture plates o...

Embodiment 6

[0072] In summary, the Lactobacillus rhamnosus of the present invention has the following characteristics:

[0073] (1) It still has a certain growth ability in the lower pH environment of pH 5.0 and 5.5, and its optimum growth pH is 6.0; the bacteria obtained by culturing at pH 6.0 can still grow in the artificial gastric juice of pH 2.0 for 3 hours. have a higher survival rate;

[0074] (2) It grows well in a temperature environment of 34-40°C, and the optimum growth temperature is 37°C;

[0075] (3) It has the effect of increasing the immune function of the human body.

Embodiment 2

[0076] Embodiment 2 prepares Lactobacillus rhamnosus preparation (liquid method control example)

[0077] 2.1 Medium

[0078] (1) MRS solid medium (for slant culture and plate culture):

[0079] Glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, agar 15g / L, K 2 HP0 4 2g / L, diammonium hydrogen citrate 2g / L, sodium acetate 5g / L, Tween-80 1g / L, MgS0 4 ·7H 2 0 0.5g / L, MnS0 4 4H 2 00.05g / L, adjust the pH to 7.0 with 1mol / L NaOH, and sterilize at 121°C for 20min.

[0080] (2) MRS liquid medium (for Erlenmeyer flask culture and fermenter culture):

[0081] Glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, tomato paste 10g / L, K 2 HP0 42g / L, diammonium hydrogen citrate 2g / L, sodium acetate 5g / L, Tween-80 1g / L, MgS0 4 ·7H 2 0 0.5g / L, MnS0 4 4H 2 00.05g / L, adjust the pH to 7.0 with 1mol / L NaOH, and sterilize at 121°C for 20min.

[0082] 2.2 Preparation method of preparation

[0083] The preparation method of the Lactobacillus rhamnosu...

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Abstract

The invention relates to a lactobacillus rhamnosus preparation capable of enhancing the immunity of a human body and a preparation method thereof. The lactobacillus rhamnosus is a lactobacillus rhamnosus MiaoTech-11 strain, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) 20862. The lactobacillus rhamnosus preparation is large in viable count and high in activity, contains metabolites which are generated by probiotics and have the effect of enhancing the immunity of the human body, and has the excellent effect of enhancing the immunity of the human body in the gastrointestinal tract of the human body.

Description

technical field [0001] The invention belongs to the technical field of probiotic production, and in particular relates to a Lactobacillus rhamnosus preparation for enhancing human immunity and a preparation method thereof. Background technique [0002] Immunity is the body's own defense mechanism, which is the body's ability to recognize and eliminate any foreign matter (viruses, bacteria, etc.) . When the human body's immune function is out of balance, or the immune system is not perfect, it is easy to cause human diseases, especially the repeated attacks of colds, tonsillitis, asthma, bronchitis, pneumonia, diarrhea and other diseases. [0003] Medical research shows that the intestinal tract is the most densely populated part of human microorganisms, and it is also the largest immune organ of the human body. 60% of the body's immune cells are located in the intestinal mucosa. The intestinal flora can promote the maturation of intestinal lymphoid tissue and the establish...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P37/04C12R1/225
CPCC12N1/20A61K35/747A61P37/04
Inventor 陈大伟童颖佳童群义陈金林吉峰
Owner 盐城维康生物科技有限公司
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