SNP (Single Nucleotide Polymorphism) marker for detecting pig eye muscle area and application thereof

A technology of eye muscle area and pig eye muscle, applied in the field of molecular biology, to achieve the effect of speeding up the pace, improving economic benefits, and increasing the speed of breeding

Active Publication Date: 2021-06-11
AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, there is still a lack of SNP markers related to pig eye muscle area that have clear functions, significant effects, and can be directly used for breeding. Therefore, it is necessary to study and explore the regulatory mechanism and genetic mechanism of pig eye muscle area in depth. Provide SNP molecular markers related to pig eye muscle area, so as to use them to breed pig breeds with high quality pork, so as to improve the theoretical and technical level of pig breeding in my country

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  • SNP (Single Nucleotide Polymorphism) marker for detecting pig eye muscle area and application thereof
  • SNP (Single Nucleotide Polymorphism) marker for detecting pig eye muscle area and application thereof

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Embodiment approach

[0037] According to a preferred embodiment of the present invention, the SNP site located at No. 134,993,242 bp on chromosome 4 of the International Porcine Genome Reference Sequence Version 10.2 is located at No. 1 of the nucleotide sequence shown in the nucleotide fragment SEQ ID NO.1 At 151bp, the difference leads to the difference in eye muscle area.

[0038] In a further preferred embodiment, the SNP marker located at 134,993,242 bp on chromosome 4 of the International Porcine Genome Reference Sequence Version 10.2 has a T / G polymorphism.

[0039] Wherein, the three genotypes corresponding to the SNP site are TT, TG and GG respectively, the TT genotype is that the bases of the two alleles of the SNP marker site are both T, and the TG genotype is the SNP The bases of the marker site in the two alleles are T and G, and the GG genotype is that the bases of the SNP marker site in the two alleles are both G.

[0040]In a further preferred embodiment, the genotype of the pig a...

Embodiment 1

[0082] 1. Experimental animals

[0083] The test pig population used in the present invention is 1173 pigs from Hebei Meishen Original Breeding Pig Farm, including 23 Duroc boars, 177 Duroc sows, 15 Landrace boars, 363 Landrace sows, and Large White male pigs. There are 2 pigs and 593 large white sows.

[0084] 2. Measurement and correction of eye muscle area

[0085] When the body weight of each pig is within the range of 85-105 kg, the eye muscle area and body weight of the individual are measured, and data such as the age of the day of measurement are recorded. The collected data were corrected for phenotypic data using the genetic evaluation trait determination procedure of the Hebei Province local standard (DB 13 / T 2065-2014) document "Technical Regulations for In-farm Production Performance Measurement of Breeding Pig Farms". At the same time, pig ear tissue samples from the test pig population were collected, placed in PBS buffer, and stored at low temperature.

[00...

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to pig eye muscle area as well as a detection method and application thereof, the SNP marker is located at the 134th, 993rd and 242nd positions of chromosome 4 of a reference sequence of version 10.2 of international pig genome and has T/G polymorphism; compared with pigs with genotypes of TT and TG, pigs with genotypes of GG of the SNP marker have a larger eye muscle area. The determination of the SNP marker related to the pig eye muscle area provided by the invention can assist in selecting pig dominant varieties with large eye muscle areas, shortens the breeding period, and improves the breeding efficiency and the breeding precision.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a SNP marker, method and application for detecting the eye muscle area of ​​pigs. Background technique [0002] my country is the largest pork production and consumption country in the world. In recent years, with the improvement of the total amount of pork and people's living standards, the demand for high-quality pork is also increasing. The eye muscle is also called the longissimus dorsi muscle. It is the freshest and tenderest meat in the whole body of a pig, and it is a representative of high-quality pork. Eye muscle area refers to the cross-sectional area of ​​longissimus dorsi, which is the main index for evaluating pig growth performance and carcass meat production capacity. The increase of eye muscle area can increase carcass lean meat rate, reduce backfat thickness, and improve feed utilization rate . [0003] Eye muscle area is a quantitative tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6806C12N15/11
CPCC12Q1/6888C12Q1/6806C12Q2600/124C12Q2600/156Y02P60/87
Inventor 唐中林陈云易国强王斌虎
Owner AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI
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