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Application of lncRNA in diagnosis and treatment of recurrent spontaneous abortion

A technology for recurrent miscarriage and products, applied in the fields of herbal medicine and molecular biology, can solve the problem of less lncRNA and achieve the effect of cycle promotion

Active Publication Date: 2021-06-15
THE SECOND HOSPITAL OF SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few studies on the role of lncRNA in recurrent miscarriage, so it is necessary to explore more effective lncRNA as a marker for RSA clinical diagnosis and treatment detection, and provide a basis for diagnosis, treatment of RSA and development of related drugs

Method used

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  • Application of lncRNA in diagnosis and treatment of recurrent spontaneous abortion
  • Application of lncRNA in diagnosis and treatment of recurrent spontaneous abortion
  • Application of lncRNA in diagnosis and treatment of recurrent spontaneous abortion

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Experimental program
Comparison scheme
Effect test

Embodiment

[0179]1. Experimental method

[0180] 1. Culture of trophoblast cell lines

[0181] The trophoblast cell lines HTR-8 / SVneo (HTR8), JAR and HEK293T cells were cultured in DMEM / F12 medium, RPMI-1640 medium and DMEM medium containing 10% FBS, respectively, at 37°C and 5% CO 2 environment in a cell culture incubator. According to the growth rate of different cells, subculture once every 1-2 days. Record the doubling time of each cell line and draw the doubling curve.

[0182] 2. Collection of clinical specimens

[0183] From September 2017 to December 2019, 25 cases of villi tissue samples (6-10 weeks) of recurrent abortion patients and 23 cases (6-10 weeks) of normal pregnancy were obtained from the Second Hospital of Shandong University. The inclusion criteria for patients with recurrent miscarriage were 2 or more consecutive miscarriages, and clear causes such as genetic defects, infection, gynecological inflammation, and tumors were excluded. The total RNA, protein and ti...

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Abstract

The invention provides application of lncRNA in diagnosis and treatment of recurrent spontaneous abortion, and belongs to the technical field of crude drugs and molecular biology. According to the invention, it is found that the expression quantity of a LINC01088 is closely related to the recurrent spontaneous abortion for the first time, and the LINC01088 has higher sensitivity and specificity in diagnosis of the recurrent spontaneous abortion, and further researches show that the LINC01088 can inhibit proliferation and invasion of trophoblasts, inhibit the cycle of the trophoblasts and promote apoptosis of the trophoblasts, thereby causing the occurrence of spontaneous abortion. Therefore, the LINC01088 and other lncRNAs are used as predictive diagnosis markers and potential treatment targets of the recurrent spontaneous abortion, play an important role in diagnosis and treatment of the recurrent spontaneous abortion, and therefore have good practical application value.

Description

technical field [0001] The invention belongs to the technical field of crude drug medicine and molecular biology, and specifically relates to the application of lncRNA in the diagnosis and treatment of recurrent miscarriage. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] According to the American Society for Reproductive Medicine, recurrent spontaneous abortion (RSA) refers to two or more consecutive embryo losses. In addition to genetic factors, abnormal uterine anatomy, infection and other clear causes, there are also recurrent miscarriages caused by unknown reasons. Due to the lack of effective clinical treatment measures, RSA has become one of the important causes o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883A61K45/00A61P15/06
CPCA61K45/00A61P15/06C12Q1/6883C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 毛海婷赵汇董娜娜李亚丽毛慧慧冯一民李宁任含笑
Owner THE SECOND HOSPITAL OF SHANDONG UNIV
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