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A kind of Bacillus albicans and its application

A bacillus and white technology, which is applied in the field of agricultural microbe technology and environmental protection, can solve the problems of single function and single variety of farmland soil, and achieve the effect of strong emission reduction, strong adaptability and growth promotion

Active Publication Date: 2022-05-17
NANJING UNIV OF INFORMATION SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the defects in the prior art that the microbial strains with soil nitrous oxide emission reduction effects are single in variety and have a single effect on farmland soil, the present invention provides a kind of Bacillus albus (Bacillus albus) Lv5A, which can effectively realize the reduction of nitrous oxide Reduce emissions while increasing crop yields

Method used

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  • A kind of Bacillus albicans and its application
  • A kind of Bacillus albicans and its application
  • A kind of Bacillus albicans and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Isolation and screening of embodiment 1 bacillus albicans Lv5A

[0029] Weigh 10g of rice rhizosphere soil (this example was sampled from rice rhizosphere soil in Dapu Town, Yixing City, Jiangsu Province in August 2018), and transfer to an ultra-clean workbench for operation. Put the soil sample into an Erlenmeyer flask filled with 90mL 0.87% NaCl solution, shake at 28°C and 200r / min for 1h, and prepare a dilution of 10 -1 soil suspension; take 10 -1 Put 10mL of the soil suspension into a conical flask filled with 90mL 0.87% NaCl solution, mix well, and make a dilution of 10 -2 soil suspension; and so on. Diluted to a dilution of 10 -3 、10 -4 、10 -5 soil suspension. Then use a pipette to pipette 10 -3 、10 -4 、10 -5 100 μL of the diluted solution was spread on the nitrogen-fixing solid medium (each liter containing KH 2 PO 4 , 0.2g; K 2 HPO 4 , 0.8g; MgSO 4 ·7H 2 O, 0.2g; CaSO 4 2H 2 O, 0.1g; FeCl 3 , trace; Na 2 Mo 4 2H 2 O, trace; yeast extract, 0....

Embodiment 2

[0030] The identification of embodiment 2 bacillus albicans Lv5A

[0031] When cultured on NBNS agar medium, the colonies are pale yellow and opaque, with rough surface, bulges, and irregular edges, and no pigmentation on various mediums; when the liquid culture is still, there is a bacterial film formation, see figure 1 . Gram staining is positive, rod-shaped, can form endophytic spores, oval in shape, blunt at both ends, sporangia are not enlarged, meso-terminal to sub-terminal, mobile; hydrolyzed starch and gelatin, acetylmethylmethanol (V-P) test was negative, nitrate reduction test was negative, phenylalanine deaminase test, indole test, methyl red (MR) test, hydrogen sulfide test were all negative.

[0032] Through 16S rDNA sequence analysis (see nucleotide sequence SEQ ID NO: 1), it is homologous to Bacillus albicans after BLAST comparison in NCBI; the physiological and biochemical characteristics are shown in Table 1 and figure 2 .

[0033] Table 1 Physiological an...

Embodiment 3

[0036] Example 3 Under different pH, the growth ability analysis of Bacillus albicans Lv5A

[0037] Inoculate 100 microliters of Bacillus albicans Lv5A bacterial solution with adjusted OD value into 10mL NBNS liquid medium with pH 4, 6, 8 and 10 respectively (volume ratio is about 1:100), at 26°C, 160r / m The shaker culture was shaken for 12, 24 and 36 hours, and the NBNS liquid medium without adding the strain was used as a control, and the growth curve of the strain was drawn by measuring the OD600 value. Three groups of repetitions were set for each group of experiments, and the results were as follows: image 3 shown.

[0038]We compared with other strains (Pseudomonas stutzeri Pseudomonas stutzeri NRCB010, Achromobacterdenitrificans NRCB029 and NRCB028), and found that Bacillus albicans Lv5A is most suitable for survival in a neutral environment, but in an alkaline environment It also has good viability in alkaline environment, and has good tolerance in alkaline environm...

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Abstract

The invention discloses a bacillus albicans and its application. The Bacillus albicans ( Bacillus albus ) Lv5A, its deposit number is CGMCC NO.21616. The Bacillus albicans isolated by the invention has strong nitrous oxide emission reduction effect, has stronger adaptability in stress environment, can effectively promote the growth of the effect, and can be widely used in rice, corn and other farmland soils.

Description

technical field [0001] The invention belongs to the field of agricultural microorganism technology and environmental protection, and relates to a strain of bacillus albicans and its application. Background technique [0002] Nitrous oxide (N 2 O), is a greenhouse gas that can be transported to the stratosphere, leading to the destruction of the ozone layer, causing the ozone hole, and because it stays in the atmosphere for a long time, and the warming potential of single molecule of nitrous oxide on the centennial scale (GWP ) is CO 2 265 times that of greenhouse gases (IPCC, 2013), the contribution to global climate change accounts for about 5-6% of the total contribution of all greenhouse gases, which has attracted worldwide attention. [0003] Agricultural soil is an important source of nitrous oxide emissions, accounting for 25-39% of the total global nitrous oxide emissions, and this proportion is still growing year by year. The large amount of nitrogen fertilizer in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C09K17/14C12R1/07
CPCC12N1/20C05G3/00C09K17/14Y02W30/40
Inventor 申卫收于心纯高南杨思琪
Owner NANJING UNIV OF INFORMATION SCI & TECH
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