Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Achromobacter denitrificans and application thereof

An achromobacter, denitrification technology, applied in the field of agricultural microbial technology and environmental protection, can solve the problems of single species and single function of farmland soil, and achieve the effect of strong emission reduction, strong adaptability, and growth promotion.

Active Publication Date: 2022-07-29
NANJING UNIV OF INFORMATION SCI & TECH
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the defects in the prior art that the microbial strains with soil nitrous oxide emission reduction effects are single in variety and have a single effect on farmland soil, the present invention provides a denitrifying Achromobacter, which can effectively reduce nitrous oxide emission, while increasing crop yields

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Achromobacter denitrificans and application thereof
  • Achromobacter denitrificans and application thereof
  • Achromobacter denitrificans and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Separation and screening of Achromobacter denitrifying bacteria YSQ030

[0025] Collect 10 g of reed rhizosphere soil from a pond in Jiangbei New District, Nanjing, Jiangsu Province, and transfer it to an ultra-clean workbench for operation. Put the soil sample into a conical flask containing 90 mL of 0.87% NaCl solution, shake at 28 °C, 200 r / min for 1 h, and prepare a dilution of 10. -1 of soil suspension; take 10 -1 10 mL of the soil suspension, put it into a conical flask containing 90 mL of 0.87% NaCl solution, mix well, and make a dilution of 10 -2 soil suspension; and so on. Diluted to 10 -3 , 10 -4 , 10 -5 soil suspension. Then use a pipette to remove 10 -3 , 10 -4 , 10 -5 100 μL of the dilution was spread on nitrogen-fixed solid medium (per liter containing KH 2 PO 4 , 0.2 g; K 2 HPO 4 , 0.8 g; MgSO 4 ·7H 2 O, 0.2 g; CaSO 4 ·2H 2 O, 0.1 g; FeCl 3 , trace; Na 2 Mo 4 ·2H 2 O, trace; yeast extract, 0.5 g; mannitol, 20 g, agar, 15 g; ...

Embodiment 2

[0026] Example 2 Identification of Achromobacter denitrifying bacteria YSQ030

[0027] The colonies were cultivated on NBNS agar medium, which were round, slightly raised, light yellow, moist, translucent, neat and smooth; figure 1 , in the figure, a is the morphological characteristics on the plate, and b is the state of the fermentation broth in liquid culture. Gram-negative, rod-shaped, spore-free, liquefied gelatin. Through 16S rDNA sequence analysis (see nucleotide sequence SEQ ID NO: 1), it is homologous to Achromobacter denitrifying after BLAST alignment in NCBI; the physiological and biochemical characteristics are shown in Table 1 and figure 2 .

[0028] Table 1 Physiological and biochemical properties of Achromobacter denitrifying bacteria YSQ030

[0029]

Embodiment 3

[0030] Example 3 Growth ability analysis of Achromobacter denitrifying bacteria YSQ030 at different pH

[0031] Inoculate 100 microliters of Achromobacter denitrifying bacteria YSQ030 with adjusted OD value into 10 mL NBNS liquid medium with pH 4, 6, 8 and 10 respectively (volume ratio is about 1:100), 26 ℃, The strains were cultured with shaking at 160 r / m for 6, 12 and 24 hours, and the NBNS liquid medium without the strain was used as a control, and the growth curve of the strain was drawn by measuring the OD600 value. Three groups of repetitions were set for each group of experiments, and the results are as follows image 3 shown. The results showed that Achromobacter denitrifying bacteria YSQ030 was most suitable for surviving in neutral environment, but also had good survivability in alkaline environment, and had better tolerance in alkaline environment.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses achromobacter denitrificans and an application thereof. The preservation number of the Achromobacter denitrificans YSQ030 is CGMCC (China General Microbiological Culture Collection Center) NO.22850, and the preservation number of the Achromobacter denitrificans YSQ030 is CGMCC NO.22850. The achromobacter denitrificans obtained by separation has a strong nitrous oxide emission reduction effect, has stronger adaptive capacity in a stress environment, can effectively promote the growth of crops, and can be widely applied to farmland soil of rice, corn and the like.

Description

technical field [0001] The invention belongs to the field of agricultural microorganism technology and environmental protection, and relates to a denitrifying Achromobacter and its application. Background technique [0002] Nitrous oxide (N 2 O), is a greenhouse gas that can be transported into the stratosphere, causing the destruction of the ozone layer, causing the ozone hole. The content of nitrous oxide in the atmosphere is very low, it is a trace gas, but it persists in the atmosphere for a long time, and the single-molecule warming potential (GWP) of nitrous oxide on the centennial scale is CO 2 265 times (IPCC, 2014), the warming effect of the increase of nitrous oxide concentration on the global climate has attracted great attention of scientists. [0003] Agricultural sources are an important source of nitrous oxide emissions, and nitrous oxide emissions from agriculture account for about 58% of the total national nitrous oxide emissions, and are increasing year b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/90C12R1/025
CPCC12N1/20C05G3/90Y02W30/40
Inventor 申卫收杨思琪高南于心纯李庆
Owner NANJING UNIV OF INFORMATION SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com