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Bacillus albus and application thereof

A bacillus and white technology, which is applied in the field of agricultural microbe technology and environmental protection, can solve the problems of single function and single variety of farmland soil, and achieve the effect of strong emission reduction, strong adaptability and growth promotion

Active Publication Date: 2021-06-29
NANJING UNIV OF INFORMATION SCI & TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the defects in the prior art that the microbial strains with soil nitrous oxide emission reduction effects are single in variety and have a single effect on farmland soil, the present invention provides a kind of Bacillus albus (Bacillus albus) Lv5A, which can effectively realize the reduction of nitrous oxide Reduce emissions while increasing crop yields

Method used

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  • Bacillus albus and application thereof
  • Bacillus albus and application thereof
  • Bacillus albus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Separation and Screening of White Bacillus LV5A

[0029] Weighed 10g of rice root soil (this example in August 2018) Sampling from the rice sluts of the Town of Yixing City, Jiangsu Province, transferred to the ultra-net workbench. The soil is placed in a tapered bottle equipped with 90 ml of 0.87% NaCl solution, oscillated at 28 ° C, 200R / min, and prepared a dilution of 10. -1 Soil suspension; take 10 -1 Soil suspension 10ml, put into a tapered bottle equipped with 90 ml of 0.87% NaCl solution, mix evenly, formulated with a dilution of 10 -2 Soil suspension; in this class. Individual composition of 10 -3 10 -4 10 -5 Soil suspension. Then remove 10 with pipettes separately -3 10 -4 10 -5 Diluent 100 μL coated in nitrogen solid medium (each containing KH 2 PO 4 0.2g; k 2 HPO 4 0.8g; MGSO 4 · 7h 2 O, 0.2g; CASO 4 · 2h 2 O, 0.1g; FECL 3 , Micro; NA 2 Mo 4 · 2h 2 O, micro; yeast extract, 0.5 g of mannitol, 20g, agar, 15g; pH 7.2, solvent is water), each gradient. In...

Embodiment 2

[0030] Example 2 Identification of White Bacillus LV5A

[0031] Cultivating the NBNS agar medium, a pale yellow opaque colony is cultured, rough surface, rumor, edge irregular, no pigment on a variety of medium; liquid culture is stationary, see figure 1 . Gram is positive, rod, can form endosporoscopy, elliptical, branger is blunt, sporestation, neutral end, sports; hydrolysis starch and gelatin, acetylmethol (VP) Test negative, nitrate reduction test negative, phenylalanine deactinase test, indole test, methyl red (MR) test, hydrogen sulfide test is negative.

[0032] Through 16S RDNA sequence analysis (see nucleotide sequence SEQ ID NO: 1), the BLAST compared with white Bacillus was homologous after the NCBI; the physiological biochemical features were shown in Table 1 and figure 2 .

[0033] Table 1 Physiological and biochemical properties of white spacine lv5a

[0034]

[0035]

Embodiment 3

[0036] Example 3 Analysis of Growth Ability of White Bacillus LV5A at different pHs

[0037] The white spiral LV5a bacterial liquid in which 100 microliters of OD values ​​was inoculated into a 10 mlNBNS liquid medium having 4, 6, 8 and 10, respectively (a volume ratio of 1: 100), 26 ° C, 160R / m. Shalled bed oscillation culture 12, 24 and 36 hours, with an NBNS liquid medium for strains, and the strain growth curve was drawn by the OD600 value. The three sets of repetitions are set each group. image 3 Indicated.

[0038]We compared with other strains (NRCB029 and NRCB028 of Pseudomonas Stutzeri Swings Neriococci NRCB010, Achromobacterdenitrificans Nitriding Nonometry), and the results of Bacillus Lv5a were found to survive at the neutral environment, but in an alkaline environment. It also has good survivability, with good tolerance in an alkaline environment, combined image 3 , Figure 4 Indicated.

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Abstract

The invention discloses bacillus albicans and application thereof. The preservation number of the bacillus albus Lv5A is CGMCC NO.21616. According to the Bacillus albus and the application thereof, the separated bacillus albus has a strong nitrous oxide emission reduction effect, has stronger adaptive capacity in a stress environment, can effectively promote the growth, and can be widely applied to farmland soil of rice, corn and the like.

Description

Technical field [0001] The present invention belongs to the field of agricultural microbial technology and environmental protection, involving a white spiralacillus and its application thereof. Background technique [0002] Nitrogen oxide (N 2 O) It is a greenhouse gas that can be delivered to the flat layer, causing the ozone layer to damage, causing ozone cavity, and because it is longer in the atmosphere, the increase of monogenic mean in the nitrid oxide (GWP) ) Is CO 2 265 times (IPCC, 2013), contribution to global climate change accounting for 5-6% of all greenhouse gases, and currently has attracted great attention worldwide. [0003] The agricultural soil is an important source of emissions of nitrid oxide, accounting for 25-39% of the total amount of nitrous oxide emissions of global oxide, and this ratio is still growing year by year. A large amount of investment in nitrogen fertilizer in agricultural production is one of the important reasons for the growth of oxidized...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C09K17/14C12R1/07
CPCC12N1/20C05G3/00C09K17/14Y02W30/40
Inventor 申卫收于心纯高南杨思琪
Owner NANJING UNIV OF INFORMATION SCI & TECH
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