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A kind of multifunctional green fluorescent carbon dot and its preparation method and application

A green fluorescent and multi-functional technology, applied in the field of fluorescent nanomaterials, can solve the problems of complex storage conditions and high costs, and achieve the effects of low preparation conditions, small toxic and side effects, and avoiding interference

Active Publication Date: 2022-07-15
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the only commercial nucleolar probe is SYTO RNASelect, which has the disadvantages of high cost and complicated storage conditions.

Method used

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  • A kind of multifunctional green fluorescent carbon dot and its preparation method and application
  • A kind of multifunctional green fluorescent carbon dot and its preparation method and application
  • A kind of multifunctional green fluorescent carbon dot and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of multifunctional green fluorescent carbon dots:

[0035] (1) Add 0.24g 3,5-diaminobenzoic acid and 0.46g citric acid to 20mL deionized water to prepare a mixed solution;

[0036] (2) transfer the mixed solution obtained in (1) into a hydrothermal reaction kettle, and perform a hydrothermal reaction at 220° C. for 3 h;

[0037] (3) the product obtained in (2) is centrifuged at 4000r / min for 20min to remove insolubles to obtain a clear solution, and after filtration and dialysis, a multifunctional green fluorescent carbon dot solution is obtained;

[0038] (4) Freeze-drying the solution of the multifunctional green fluorescent carbon dots obtained in (3) to obtain the multifunctional green fluorescent carbon dots.

[0039] The photos of the prepared multifunctional green fluorescent carbon dot solution under the excitation of fluorescent lamp and wavelength of 420 nm, respectively, are shown in figure 1 , the left picture is the picture of the multifunctio...

Embodiment 2

[0046] Preparation of multifunctional green fluorescent carbon dots:

[0047] (1) Add 0.24g 3,5-diaminobenzoic acid and 0.46g citric acid to 30mL deionized water to prepare a mixed solution;

[0048] (2) The mixed solution obtained in (1) was transferred to a hydrothermal reaction kettle, and hydrothermally reacted at 220° C. for 5 h;

[0049] (3) the product obtained in (2) is centrifuged at 4000r / min for 20min to remove insolubles to obtain a clear solution, and after filtration and dialysis, a multifunctional green fluorescent carbon dot solution is obtained;

[0050] (4) Freeze-drying the solution of the multifunctional green fluorescent carbon dots obtained in (3) to obtain the multifunctional green fluorescent carbon dots.

Embodiment 3

[0052] Preparation of multifunctional green fluorescent carbon dots:

[0053] (1) Add 0.24g 3,5-diaminobenzoic acid and 0.46g citric acid to 20mL deionized water to prepare a mixed solution;

[0054] (2) The mixed solution obtained in (1) was transferred to a hydrothermal reaction kettle, and hydrothermally reacted at 190° C. for 5 h;

[0055] (3) the product obtained in (2) is centrifuged at 4000r / min for 20min to remove insolubles to obtain a clear solution, and after filtration and dialysis, a multifunctional green fluorescent carbon dot solution is obtained;

[0056] (4) Freeze-drying the solution of the multifunctional green fluorescent carbon dots obtained in (3) to obtain the multifunctional green fluorescent carbon dots.

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Abstract

The invention provides a multifunctional green fluorescent carbon dot, a preparation method and application thereof, and belongs to the field of fluorescent nanometer material preparation. Preparation steps of carbon dots: 3,5-diaminobenzoic acid and citric acid are dissolved in secondary water and transferred to a hydrothermal reactor for hydrothermal reaction; the obtained product is centrifuged to remove insolubles, and dialysis to remove impurities to obtain a multifunctional The green fluorescent carbon dot solution is freeze-dried to obtain multifunctional green fluorescent carbon dots. The invention has simple process and low requirements for preparation conditions; and the prepared multifunctional green fluorescent carbon dots have low toxicity and high fluorescence quantum yield. The prepared multifunctional green fluorescent carbon dots can be applied to NO in cells 2 ‑ Detection, pH Sensing and Nucleolar Targeting and NO in Aqueous Solutions 2 ‑ Colorimetric identification.

Description

technical field [0001] The invention relates to fluorescent nanomaterials, in particular to a multifunctional green fluorescent carbon dot and a preparation method thereof, as well as applying the multifunctional green fluorescent carbon dot to NO in cells 2 - Detection, pH Sensing and Nucleolar Targeting and NO in Aqueous Solutions 2 - Colorimetric identification. Background technique [0002] In 2004, Xu et al. (J.Am.Chem.Soc., 2004, 126, 12736-12737) first reported the accidental discovery of carbon dots during the separation and purification of single-walled carbon nanotubes. In 2006, Sun et al. (J.Am.Chem.Soc., 2006, 128, 7756-7757) synthesized fluorescent carbon dots with excellent performance by means of laser etching and surface passivation. Since then, carbon dots have become a good alternative to traditional semiconductor quantum dots due to their tunable fluorescence, excellent photostability, good water solubility, low toxicity, and excellent biocompatibility...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B32/15C09K11/65B82Y20/00B82Y40/00
CPCC01B32/15C09K11/65B82Y20/00B82Y40/00
Inventor 石利红王倩靓
Owner SHANXI UNIV