Fumosoroseanoside B capable of resisting bacteria, scavenging free radicals and inhibiting tyrosinase activity and preparation method of fumosoroseanoside B

A technology of tyrosinase and nicotinoid, which is applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of preparation, identification and biological activity research of nicotinoid compounds that have not yet been seen. reports, etc.

Active Publication Date: 2021-07-06
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the long-term co-evolution with insects, this type of fungus can produce a large number of new bioactive compounds. However, there are still few studies on the metabolites of this type of fungus. to report

Method used

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  • Fumosoroseanoside B capable of resisting bacteria, scavenging free radicals and inhibiting tyrosinase activity and preparation method of fumosoroseanoside B
  • Fumosoroseanoside B capable of resisting bacteria, scavenging free radicals and inhibiting tyrosinase activity and preparation method of fumosoroseanoside B
  • Fumosoroseanoside B capable of resisting bacteria, scavenging free radicals and inhibiting tyrosinase activity and preparation method of fumosoroseanoside B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The preparation steps of macaridin B having antibacterial removal radicals and inhibiting tyrosinase activity are as follows:

[0052] (1) Preparation of solid cultures

[0053] 1.1 culture primary strains

[0054] Water saucer will be saved ( Cordyceps fumosorosea The hydrolyzed strain is inoculated in potato agar (PDA) beveled medium, and 10 μl of each slope is inoculated, at a temperature of 20 ° C, culture 5d, and collecting spores to obtain a primary strain;

[0055] The PDA medium, is a potato medium, which formulas: 6g potato dry powder, 20 g of glucose, 15g agar, and water to 1000 mL.

[0056] 1.2 cultured secondary strains

[0057] Embel aimed with water with water 5 ~ 10 7 A spore / ml of spore suspension, press 5 μL per plate to in advance (9 × 100 mm in the PDA plate), at 20 ° C, thermostatic culture for 6 days, collected spores, 5 degree strains . The PDA medium composition is in the same step (1).

[0058] 1.3 three-stage solid expanded culture

[0059] Substa...

Embodiment 2

[0070] The preparation steps of macaridin B having antibacterial removal radicals and inhibiting tyrosinase activity are as follows:

[0071] (1) Preparation of solid cultures

[0072] 1.1 primary strain culture

[0073] Water saucer will be saved ( Cordyceps fumosorosea The hydrolyzed strain is inoculated in potato agar (PDA) bevel medium, and 30 μl of each slope is inoculated, at a temperature of 30 ° C, culture 10d, and collecting spores to obtain a primary strain;

[0074] The PDA medium, is a potato medium, which formulas: 6g potato dry powder, 20 g of glucose, 15g agar, and water to 1000 mL.

[0075] 1.2 secondary strain culture

[0076] Embel aimed with water with water 7 A spore / ml of spore suspension, pressing 400 μL per plate to a PDA plate (9 × 100 mm in the PDA plate) per plate (9 × 100 mm), 30 ° C, constant temperature culture for 12 days, collected spores, to obtain a secondary strain . The PDA medium composition is in the same step (1.1).

[0077] 1.3 three-stage ...

Embodiment 3

[0089] The preparation steps of macaridin B having antibacterial removal radicals and inhibiting tyrosinase activity are as follows:

[0090] (1) Preparation of solid cultures

[0091] 1.1 primary strain culture

[0092] Water saucer will be saved ( Cordyceps fumosorosea The hydrolyzed strain is inoculated into potato agar (PDA) bevel medium, 20 μl of each slope inoculated, at temperature 25 ° C, culture 8d, and collected spores to obtain a primary strain;

[0093] The PDA medium, is a potato medium, which formulas: 6g potato dry powder, 20 g of glucose, 15g agar, and water to 1000 mL.

[0094] 1.2 secondary strain culture

[0095] Embel aimed with water with water 6 A spore / ml of spore suspension, in accordance with 300 μl of each plate to PDA plates (9 × 100 mM of the PDA plate) per plate (9 × 100 mM), 10 days of 10 days, and the secondary strain was collected by 10 days at 25 ° C, thermostat culture. The PDA medium composition is in the same step (1.1).

[0096] 1.3 three-sta...

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Abstract

The invention relates to fumosoroseanoside B capable of resisting bacteria, scavenging free radicals and inhibiting tyrosinase activity and a preparation method of the fumosoroseanoside B, and belongs to the technical field of extraction and preparation of biological products. The fumosoroseanoside B is light yellow brown powder, is a novel compound, and is not reported in literatures yet. The compound provided by the invention can be obtained by culturing the cordyceps fumosorosea and then extracting and separating the cordyceps fumosorosea. The pure fumosoroseanoside B is light yellow brown powder, and when the concentration of the fumosoroseanoside B is 200 mg / mL, the inhibition zone of the fumosoroseanoside B on escherichia coli is 17.3 mm, and the inhibition zone of the fumosoroseanoside B on streptococcus albus is 19.8 mm; the half clearance concentration (DC50) of the diphenylpicryl phenylhydrazine free radicals (DPPH) is 345 [mu] g / mL; and the median inhibitory concentration (IC50) on tyrosinase is 106 [mu] g / m. The compound has the potential of preparing antibacterial and antifungal drugs and has the potential of preparing antioxidant, whitening and anti-aging health care products.

Description

Technical field [0001] The present invention belongs to the technical field of extraction preparation of biological products, and is relates to extraction, purification, structural identification and activity assays having anti-oxidation, antibacterial and tyrosin inhibitory activity. Background technique [0002] Macroide B is from a moulderlutine bacterium Cordyceps fumosorosea Novel glycoside substances obtained by separating and prepared in the culture, with antibacterial, antifungal, removal of free radicals and tyrosinase inhibition. [0003] Nothive type of macarrus exists in the soil and parasitic in insects, before the miseoholic coridine ( Isariafumosorosea ) Or Macro-proposed pupil ( Paecilomycesfumosorosea ). The bacteria hosts are widely used, susceptible to aphids, flour, beetle, termites, wasps, and a variety of lepidoptera, so macarrothoacteria is an important biological control fungi. This type of fungi can produce a large number of new biological active compound...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/06C12P19/60C12N1/14C12R1/645
CPCC07H17/04C07H1/06C12P19/60C12N1/14Y02A50/30
Inventor 陆瑞利胡丰林周雪赵铖
Owner ANHUI AGRICULTURAL UNIVERSITY
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