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Glycosylated hemoglobin detection kit

A technology for glycated hemoglobin and detection kit, which is applied in the field of glycated hemoglobin detection, can solve the problems of limited particle enlargement, large difference in results, insufficient detection sensitivity, etc., and achieves the effect of stable long-term storage

Pending Publication Date: 2021-07-16
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the secondary antibody has only two sites to bind to the glycosylated hemoglobin monoclonal antibody, the particle size of "latex microspheres-glycosylated hemoglobin-glycosylated hemoglobin monoclonal antibody-secondary antibody" formed in the detection is limited, and the detection sensitivity is not enough
Moreover, because the glycosylated hemoglobin antibody and the secondary antibody coexist in the same system for a long time, the test may be unstable. On-site mixed inspection by inspectors
This increases operator error, leading to large variances in results

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0047] Preparation of glycosylated hemoglobin detection kit

[0048] All the components shown in Table 1 below were mixed at the concentrations shown below to prepare the first reagent and the second reagent.

[0049] Table 1

[0050]

[0051]

Embodiment 2

[0053] Preparation of glycosylated hemoglobin detection kit

[0054] All the components shown in Table 2 below were mixed at the concentrations shown below to prepare the first reagent and the second reagent.

[0055] Table 2

[0056] first reagent components concentration 120nm latex microspheres 0.1％ Glycine buffer 50mmol / L borate buffer 20mmol / L TWEEN 20 0.2％ second reagent Glycosylated hemoglobin monoclonal antibody 0.2mg / mL Glycine buffer 20mmol / L borate buffer 20mmol / L TWEEN 20 0.08％ Protein A 0.1mg / mL

Embodiment 3

[0058] Preparation of glycosylated hemoglobin detection kit

[0059] All the components shown in Table 3 below were mixed at the concentrations shown below to prepare the first reagent and the second reagent.

[0060] table 3

[0061] first reagent components concentration Particle size 80nm latex microspheres 0.08％ Glycine buffer 20mmol / L borate buffer 50mmol / L TWEEN 20 0.1％ second reagent Glycosylated hemoglobin monoclonal antibody 0.1mg / mL Glycine buffer 20mmol / L borate buffer 20mmol / L TWEEN 20 0.05％ Protein G 0.1mg / mL

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Abstract

The invention relates to the technical field of glycosylated hemoglobin detection, and particularly discloses a glycosylated hemoglobin detection kit which comprises a first reagent and a second reagent. The first reagent comprises latex microspheres, a first buffer solution and a first surfactant; and the second reagent comprises a protein A or a protein G, a glycosylated hemoglobin monoclonal antibody, a second buffer solution and a second surfactant. Through the mode, the kit disclosed by the invention can be stably stored for a long time, and the tedious operation that a monoclonal antibody and a secondary antibody of a reagent in the current market need to be respectively stored and are uniformly mixed for use in the use period is avoided.

Description

technical field [0001] The present application relates to the technical field of detection of glycosylated hemoglobin, in particular to a detection kit for glycosylated hemoglobin. Background technique [0002] Existing detection methods for glycosylated hemoglobin include ion exchange chromatography, high performance liquid chromatography, affinity chromatography, radioimmunoassay, enzyme immunoassay and latex microsphere immunoturbidimetric method. The current common latex microsphere immunoturbidimetric direct method assay kit usually consists of three reagent components. The detection principle is: use blank latex microspheres to absorb the hemoglobin in the test sample, then add the glycosylated hemoglobin monoclonal antibody that specifically recognizes glycosylated hemoglobin (HbA1c) and the corresponding secondary antibody (usually the secondary antibody is goat / rabbit anti-mouse glycosylated hemoglobin monoclonal antibody), form antigen-antibody complexes (Complexe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72G01N33/577
CPCG01N33/72G01N33/577
Inventor 马志亚陆锋
Owner SHENZHEN DYMIND BIOTECH
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