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Gene sequencing signal crosstalk removal method

A gene sequencing and signal technology, applied in the field of gene sequencing, can solve the problems of low signal intensity and low signal intensity, etc.

Pending Publication Date: 2021-07-20
CYGNUS BIOSCI BEIJING CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Generally, due to the relatively low signal intensity of sequencing, the signal intensity of each data point of gene sequencing is not very high

Method used

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  • Gene sequencing signal crosstalk removal method
  • Gene sequencing signal crosstalk removal method
  • Gene sequencing signal crosstalk removal method

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0097] In this embodiment, the pit positions are distributed at equal intervals on the two-dimensional plane, and the pit pitch DL=8 pixels.

[0098] 1. Extraction Strength

[0099] Depending on pit location and size, such as Figure 6 As shown, draw a series of circles (can also be in the shape of a rectangle), and calculate the average value of all pixels in each circle as the signal intensity of the pit. Since the pits are distributed horizontally and vertically on the chip, the intensity matrix I can be obtained according to the geometric position distribution of the pits, and each element value of the matrix represents the intensity value of a certain pit.

[0100] When calculating the signal strength, since the general micro-pit is circular, the position of the micro-pit is delineated by drawing a circle. The method can also be changed when the dimples are of other shapes.

[0101] When calculating the signal strength of four adjacent micropits, the adjacent non-circu...

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Abstract

The invention provides a method for removing crosstalk of gene sequencing signals. And measuring and calculating by using isolated data points, and finally obtaining a real sequencing signal in a complex environment. The method is suitable for a sequencing method with the three ends not closed. The crosstalk method comprises the following steps: (1) sequencing to obtain a sequencing result image; (2) collecting the signal intensity of the sequencing micro-pit; (3) setting a signal intensity threshold value; (4) screening out isolated bright pits; (5) estimating crosstalk; and (6) applying the crosstalk average value to obtain a corrected sequencing signal strength value.

Description

technical field [0001] The invention relates to a method for removing crosstalk from gene sequencing and sequencing signals, and belongs to the field of gene sequencing. Background technique [0002] Gene sequencing is an industry that has developed rapidly in recent years. The process of gene sequencing is actually a process of synthesis of double-stranded DNA molecules. In high-throughput gene sequencing, due to its extremely low signal intensity and extremely small micropits, the reaction is generally performed within the range of 0.2-2 microns, so the repeatability of the signal is not strict. In other words, in the process of high-throughput sequencing, the repeatability and consistency of signals will not be perfect. In this case, the processing of sequencing signals is an important way to improve the quality of sequencing. In modern signal processing, there may be many techniques that can do this relatively simple signal. However, due to its uniqueness, gene seque...

Claims

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Application Information

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IPC IPC(8): G16B30/10G16B40/00
CPCG16B30/10G16B40/00
Inventor 陈子天周文雄黄家蔚段海峰李文涛
Owner CYGNUS BIOSCI BEIJING CO LTD