Application of Talinum paniculatum (Jacq.) Gaertn. polysaccharide on aspect of accelerating dissolving of baicalin in water, and method
The technology of red ginseng polysaccharide and ginseng polysaccharide is applied in the application field of red ginseng polysaccharide in promoting the dissolution of baicalin in water, and can solve the problems of intestinal wall damage, hidden danger of medication safety, leakage of phospholipid compound drugs, etc., and achieves broad application prospects , The effect of improving solubility and high development value
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Examples
Embodiment 2
[0047] Example 2 Red ginseng polysaccharide increased the degree of solubility of baicalin composition
[0048] Determination of balanced solubility: Take ionin 2mg in 2 ml of centrifuge tube, 2 parts of parallel, add 1 ml of concentration of 5% red ginseng polysaccharide solution (with pure water as blank solvent), vortex 2min, centrifuge, and place in 37 Cove in the rice cooker in the rice cooker 24h so that it fully reaches the balance. The sample was taken off to room temperature, and 12000 rpm was centrifuged for 10 min, precision absorption of 0.2 mL of the supernatant, placed in a new 2 ml of centrifuge tube, add 0.8 ml of methanol to precipitate polysaccharide, and the polysaccharide is completely precipitated, 12000 rpm Centrifuge 10 min, 12000 rpm Take the supernatant, a 0.45 μm filter, injected into HPLC, and assay.
[0049] Solubility assay: Column: Agilent Eclipse XDB C18 (4.6 * 250mm, 5μm); mobile phase: A-methanol, B-0.1% phosphoric acid; flow rate: 1 mL / min, colu...
Embodiment 3
[0053] Effect of Example 3 Red Paramemic Polysaccharide with Polysin Comparisons on Solubility
[0054] like Image 6 The baicalin component 2 mg in 2 ml of centrifuge tube, according to the baicide: polysaccharide ratio of 1: 160, 1: 80, 1: 40, 1: 20, 1: 10, 1: 5, 1: 2.5, 1: 1.25, 1: 0.625, 1: 0.3125 Add red gamfrangosaccharide, (with pure water as a white solvent), vortex 2min, the centrifuge is sealed in a 37 ° C water bath, so that it makes it sufficient to equilibrate. The sample was taken off to room temperature, and 12000 rpm was centrifuged for 10 min, precision absorption of 0.2 mL of the supernatant, placed in a new 2 ml of centrifuge tube, add 0.8 ml of methanol to precipitate polysaccharide, and the polysaccharide is completely precipitated, 12000 rpm Centrifuge 10 min, 12000 rpm Take the supernatant, a 0.45 μm filter, injected into HPLC, and assay.
[0055] The results showed that the solubility of the baicalin increased significantly with the ratio of the polysacchari...
Embodiment 4
[0056] Effect of Example 4 Red Paramemic Polysaccharide on Stability of Baricalin Composition
[0057] In order to assess the stability of individual baicalins and BAI-RGP (1: 5, 1: 10, 1: 20), three different pH (1.2, 6.8 and 8.3) are used to simulate stomach, small intestine and large intestine. environment. 2 mg of baicalin was placed in a 10 ml brown volumetric flask, and then pH 1.2, pH 6.8 or pH 8.3 was added, vortex for 2 minutes, and the initial concentration was determined using the established HPLC method (C 0 ). For BAI-RGP group, 2 mg of baicalin and corresponding amount of RGP is added, and the remaining concentration is measured at different time points (C t ). Related parameters of baicalin and BAI-RGP in different pH conditions, including table optometry constants (kobs), very unsained period (T 0.9 ) And half-life (T 0.5 ), Chemical dynamics equation.
[0058] The result shows that Figure 8 And Table 2 shows, Time (T) is in (C t / C 0 ) Linear regression gains deg...
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Claims
Application Information
- IPC
- A61K47/36; A61K31/7048; A61K36/539; A61P29/00; A61P39/06; A61P35/00; A61P31/12; C08B37/00
- CPC
- A61K47/36; A61K31/7048; A61K36/539; A61P29/00; A61P39/06; A61P35/00; A61P31/12; C08B37/006
- Inventors
- 贾晓斌; 廉源沛