Construction method of ratio fluorescent nano sensor for Ag < + > sensitive detection
A nano-sensor and construction method technology, applied in chemical instruments and methods, fluorescence/phosphorescence, instruments, etc., can solve the problems of expensive instruments and equipment, high detection costs, complicated operations, etc., to improve detection accuracy and simplify construction process. , the effect of simple preparation process
Active Publication Date: 2021-07-27
JIANGSU UNIV
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Although these methods have high precision and low detection limit, their use is greatly li
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Embodiment 1
[0043] (1) Preparation of internal standard fluorescent probe NAC-CDZNTE QDS
[0044] 0.0511 g of 粉 (TE) and 0.0757 g of sodium borohydride (NABH 4 Dissolved in 20 ml of ultrapure water, nitrogen gas 10min; then, while nitrogen gas, while stirring at 80 ° C, the reaction was stirred for 30 min, and the purple NaHTE solution was obtained. 0.0914 g cadmium chloride (CDCL 2 · 2.5H 2 O), 0.0055 g zinc chloride (ZnCl 2 ), 0.1110 g of N-acetyl-L-cysteine (C 5 Hide 9 NO 3 S) dissolved in 80 ml of ultra-pure water, adjusted to pH = 9 with a 1m sodium hydroxide solution (NaOH), 5 min. Then, the freshly prepared 4 ml NaHte solution was rapidly added to the above mixed liquid, mixed uniform and continued 5 min. Finally, the mixture was transferred to the polytetrafluoroethylene stainless steel reaction kettle, and the reaction was reacted at 190 ° C for 43min; after the reaction was completed, then centrifugally washed several times, redistributed in 30 ml of ultra-pure water, 4 ° C preser...
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The invention belongs to the field of fluorescence analysis, and discloses a construction method of a ratio fluorescence nano sensor for Ag < + > sensitive detection. The method is characterized in that the ratio fluorescent nano sensor is prepared by mixing two stable quantum dots (QDs), rapid and sensitive detection of Ag < + > is realized, the ratio of Ag < + > concentration to fluorescence intensity presents a good linear relationship in a concentration interval of 0.5-4 [mu] M, and the detection limit can reach 0.17 [mu] M. By adopting a ratio fluorescence method, the influence caused by detection background fluctuation is effectively avoided, and the detection accuracy is improved. The non-closed NAC-CdZnTe QDs are used as the internal standard probe, the construction process of the fluorescence sensor is simplified, and the Lcys-CdZnTe QDs are used as the signal fluorescence probe and have specific response to Ag < + >. The two QDs have good water solubility, and the sensor only needs to be shaken up in the construction process, so that the operation is simple and convenient. The ratio fluorescent nano sensor provided by the invention has the characteristics of simple preparation process, short time consumption in the detection process, small reagent dosage, low detection cost and the like.
Description
Technical field [0001] The present invention belongs to the field of nanomaterial fluorescence analysis, characterized by mixing two stable quantum dots (QDS) preparation ratio fluorescent nanosensor, realization of AG + Fast, sensitive detection. Background technique [0002] Silver and its compounds are widely used in electronics, imaging, optical, batteries, and pharmaceuticals. In addition, silver antibacterial effect is remarkable, and can be used in life supplies such as antibacterial agents, daily detergents and cosmetics. AG + At low concentrations, it has good antibacterial activity, as well as strong inhibition and sterilization. Low concentration AG + It has bactericidal and antibacterial effect in drinking water. However, AG + It is listed as one of the strongest toxicity in heavy metal ions. Although not biogens accumulatory toxins, AG + It is capable of inactivating mercaptozymes with an amine group, imidazole, and carboxyl group of various metabolites, and can repl...
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Login to View More IPC IPC(8): G01N21/64C09K11/88C09K11/02
CPCG01N21/643C09K11/883C09K11/025G01N2021/6432
Inventor 钱静崔海宁王坤王成全贾素莉
Owner JIANGSU UNIV