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Composite probiotic preparation for improving production performance of sows and preparation method of composite probiotic preparation

A technology of compound probiotics and production performance, applied in microorganism-based methods, biochemical equipment and methods, bacteria used in food preparation, etc. problems, to achieve the effect of normal color, improved immunity, and reduced degree of diarrhea

Pending Publication Date: 2021-07-30
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, most of the existing technologies are fermented traditional Chinese medicine feed additives or premixes containing probiotics, etc., which not only have a single type of object, but also a single growth stage, and lactic acid bacteria are not resistant to high temperatures, and will lose their activity during the high-temperature granulation process , the effect is not as expected

Method used

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  • Composite probiotic preparation for improving production performance of sows and preparation method of composite probiotic preparation
  • Composite probiotic preparation for improving production performance of sows and preparation method of composite probiotic preparation
  • Composite probiotic preparation for improving production performance of sows and preparation method of composite probiotic preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Pediococcus lactis PA-19 acid resistance, bile salt resistance and its antibacterial properties experiment

[0077] The cryopreserved Pediococcus lactis PA-19 was inoculated in the MRS liquid medium, cultured statically at a temperature of 37°C for 18 hours, and subcultured twice in this way to obtain an activated fermentation broth;

[0078] The composition of the MRS liquid medium is as follows: 10g peptone, 5g beef extract, 4g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1mL Tween 80, 0.2g magnesium sulfate, Add 0.05g of manganese sulfate to 1000mL of distilled water, adjust the pH to 6.5, and sterilize at 121°C for 15min.

[0079] (1) Detection of acid resistance and bile salt resistance

[0080] Add 3.5g / L pepsin to the sterilized PBS buffer at pH 2.5 (adjusted with 1mol / L HCl), filter and sterilize with a 0.22μm microporous membrane to make simulated gastric juice; centrifuge the activa...

Embodiment 2

[0094] Embodiment 2 is used to improve the preparation of the compound probiotic preparation of sow production performance

[0095] Preparation of Lactobacillus plantarum P-8 bacterial agent, Lactobacillus plantarum KT-Lp9 bacterial agent, Pediococcus lactis PA-19 bacterial agent:

[0096] Separately carry out high-density fermentation of 3 strains of fermentation strains: take one ring of activated Lactobacillus plantarum P-8, Lactobacillus plantarum KT-Lp9, and Pediococcus lactis PA-19 respectively, and inoculate them into MRS respectively In the culture medium, all at a temperature of 37°C, all cultivated for 24h under the condition of a rotating speed of 85rpm, respectively to obtain a first-class seed liquid; the cultivated first-class seed liquid was transferred again according to the inoculum size of 7% (v / v). Put into MRS culture medium to carry out secondary activation, after activating 24h, obtain secondary seed liquid; Insert secondary seed liquid into different f...

Embodiment 3

[0101] Embodiment 3 compound probiotic preparation effect experimental example

[0102] In the pig production base, the compound probiotic preparation prepared in Example 2 was added to the basal ration at an amount of 5 kg / ton of ration.

[0103] The production base is: Inner Mongolia Zhengda Food Co., Ltd. Zhengyuan 5,000 kinds of pig farms;

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Abstract

The invention discloses a compound probiotic preparation for improving the production performance of sows and a preparation method and an application thereof. The compound probiotic preparation comprises lactobacillus plantarum P-8, lactobacillus plantarum KT-Lp9, pediococcus acidilactici PA-19 and metabolites of the lactobacillus plantarum P-8, the lactobacillus plantarum KT-Lp9 and the pediococcus acidilactici PA-19, wherein the microbial preservation number of the lactobacillus plantarum P-8 is CGMCC No.6312, and the microbial preservation number of the lactobacillus plantarum KT-Lp9 is CGMCC No.12950; and the microbial preservation number of pediococcus acidilactici PA-19 is CGMCC No.19881. The composite probiotic preparation is prepared by adopting a method of respectively fermenting and then mixing, can be widely applied to pigs of different types and different growth stages, and still has an ideal effect after high-temperature granulation.

Description

technical field [0001] The application belongs to the field of probiotic preparations, in particular to a compound probiotic preparation for improving the production performance of sows and a preparation method thereof. Background technique [0002] Antibiotics have played a huge role in the prevention and treatment of animal diseases, the promotion of animal growth, the improvement of feed utilization, the improvement of the economic benefits of farmers, and the acceleration of the development of animal husbandry in the animal husbandry industry. However, the long-term use of antibiotics leads to the decline of animal immunity, the emergence of drug resistance of pathogenic bacteria, drug residues in animal products, environmental pollution, and serious harm to human health and living environment. After the prohibition of adding antibiotics in feed, the pig farming industry is facing major farming problems. Therefore, it is imminent to find a method that can effectively pr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/30A23K10/18C12R1/25C12R1/01
CPCC12N1/20A23K50/30A23K10/18A23V2400/413A23V2400/169Y02P60/87
Inventor 高旭黄卫强于海静
Owner INNER MONGOLIA SCI PLUS BIOTECH
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