Composite probiotic preparation for improving production performance of sows and preparation method of composite probiotic preparation

A technology of compound probiotics and production performance, applied in microorganism-based methods, biochemical equipment and methods, bacteria used in food preparation, etc. problems, to achieve the effect of normal color, improved immunity, and reduced degree of diarrhea

Pending Publication Date: 2021-07-30
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, most of the existing technologies are fermented traditional Chinese medicine feed additives or premixes containing probiotics, etc., which are not only suitable for a single type of object, but

Method used

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  • Composite probiotic preparation for improving production performance of sows and preparation method of composite probiotic preparation
  • Composite probiotic preparation for improving production performance of sows and preparation method of composite probiotic preparation
  • Composite probiotic preparation for improving production performance of sows and preparation method of composite probiotic preparation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0076] Example 1 Lactic acid chippenti PA-19 acid, aspirational salt characteristics and antibacterial characteristics

[0077] The frozen sauces were inoculated in the MRS liquid medium, static in the temperature of 37 ° C for 18 h, such that biochemistry was cultured for 2 times to obtain an activated fermentation liquid;

[0078] The MRS liquid medium composition is as follows: 10g protein, 5g beef cream, 4G yeast powder, 20 g glucose, 2g hydrogen phosphate, sodium acetate, trisodium citrate, 1 mL Tween 80, 0.2 g of magnesium sulfate, 0.05 g of manganese sulfate was added 1000 mL of distilled water to adjust the pH to 6.5, 121 ° C for 15 min.

[0079] (1) Detection of acid resistance and aspiration

[0080] In pH 2.5 (adjusted by 1 mol / L HCl) Sterilization PBS buffer, 3.5 g / L gastric protease was added, and the sterilization was filtered with 0.22 μm microporous filtration membrane, and the gastric liquid was mounted; the activated strain centrifuge The bacteria was collec...

Example Embodiment

[0094] Example 2 Preparation of a compound probiotic formulation for improving the production of sow

[0095] Plant Lactobacillus P-8 Fungicide, Plant Lactobacillus Kt-LP9 Fungicide, Preparation of Pelica PA-19 Fungicides:

[0096] 3 strains of fermentation bacteria were separately fermented separately: Taken a ring activated plant Lactobacillus P-8, plant Lactobacillus KT-LP9, lactic acid chippenti PA-19, respectively, each inoculated to MRS, respectively. In the medium, it was cultured at a temperature of 37 ° C, both at a rotational speed of 85 rpm, and obtained a grade of seed fluid; a cultured primary seed fluid was re-transferred separately by 7% (V / V). The secondary activation was carried out in the MRS medium, and the secondary seed liquid was obtained after 24 hours; the secondary seed fluid was each accessible to different fermentor medium according to the same inoculation amount of 7% (V / V), respectively, and temperature It was 37 ° C, the rotational speed was 85 r...

Example Embodiment

[0101] Example 3 Experimental Experimental Experimental Experimental Effect of Composite Probiotics

[0102] In the pig production base, the compound probiotic bacteria preparation prepared in Example 2 was added to the base diet, and the amount was 5 kg / ton day grain.

[0103] The production base is: 5,000 pig farms in the front edge of Inner Mongolia Zhengda Food Co., Ltd .;

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Abstract

The invention discloses a compound probiotic preparation for improving the production performance of sows and a preparation method and an application thereof. The compound probiotic preparation comprises lactobacillus plantarum P-8, lactobacillus plantarum KT-Lp9, pediococcus acidilactici PA-19 and metabolites of the lactobacillus plantarum P-8, the lactobacillus plantarum KT-Lp9 and the pediococcus acidilactici PA-19, wherein the microbial preservation number of the lactobacillus plantarum P-8 is CGMCC No.6312, and the microbial preservation number of the lactobacillus plantarum KT-Lp9 is CGMCC No.12950; and the microbial preservation number of pediococcus acidilactici PA-19 is CGMCC No.19881. The composite probiotic preparation is prepared by adopting a method of respectively fermenting and then mixing, can be widely applied to pigs of different types and different growth stages, and still has an ideal effect after high-temperature granulation.

Description

technical field [0001] The application belongs to the field of probiotic preparations, in particular to a compound probiotic preparation for improving the production performance of sows and a preparation method thereof. Background technique [0002] Antibiotics have played a huge role in the prevention and treatment of animal diseases, the promotion of animal growth, the improvement of feed utilization, the improvement of the economic benefits of farmers, and the acceleration of the development of animal husbandry in the animal husbandry industry. However, the long-term use of antibiotics leads to the decline of animal immunity, the emergence of drug resistance of pathogenic bacteria, drug residues in animal products, environmental pollution, and serious harm to human health and living environment. After the prohibition of adding antibiotics in feed, the pig farming industry is facing major farming problems. Therefore, it is imminent to find a method that can effectively pr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/30A23K10/18C12R1/25C12R1/01
CPCC12N1/20A23K50/30A23K10/18A23V2400/413A23V2400/169Y02P60/87
Inventor 高旭黄卫强于海静
Owner INNER MONGOLIA SCI PLUS BIOTECH
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