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Quantitative determination method for residual quantity of quizalofop-p-tefuryl and sulcotrione in pig muscle and kidney tissue
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A technology for the quantitative determination of sulcotrione, which is applied in the field of determination of pesticide residues, can solve the problems of no retrieval of glycyrrhizin and no detection method, etc.
Pending Publication Date: 2021-07-30
ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Sulcotrione has been included in my country's national mandatory standard GB 2763-2019 "National Food Safety Standard Maximum Residue Limits of Pesticides in Food", but there is no relevant detection method, and no corresponding method for the determination of residues in food has been found for glycyrrhizin.
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Embodiment 1
[0032] Example 1: Detection of glycoside and sulcotrione residues in pig kidney tissue:
[0033] Step 1: Take about 500g of pig kidney tissue, pulverize it evenly with a tissue grinder, put it into a clean container and seal it, and mark it. Weigh 2g of the sample, put it into a 50mL centrifuge tube, add 5mL of water, add 20mL of acidified acetonitrile, sonicate for 6min, add 2g of anhydroussodiumsulfate, vortex for 1min, centrifuge at 4500r / min for 5min, and remove the supernatant after centrifugation for later use.
[0034] Step 2: Accurately draw 10mL of the subnatant into a 15mL graduated centrifuge tube, add 850mg of anhydrousmagnesiumsulfate, 100mg of PSA, and 150mg of C18, vortex for 1.0min, centrifuge at 5000r / min for 5min, and draw 1mL of the supernatant through a 0.2μm microporous filter Membrane, to obtain sample purification solution, to be determined by LC-MS / MS.
[0035] Step 3: Preparation of standard working solutions of sugar glufosinate and sulcotrione. ...
Embodiment 2
[0043] Example 2: Detection of sugar glucoside and sulcotrione residues in pig muscle tissue:
[0044] Step 1: Take about 500g of pig muscle tissue, pulverize it evenly with a tissue grinder, put it into a clean container and seal it, and mark it. Weigh 2g of the sample, put it into a 50mL centrifuge tube, add 5mL of water, add 25mL of acidified acetonitrile, ultrasonicate for 8min, add 3g of anhydroussodiumsulfate, vortex for 1min, centrifuge at 4500r / min for 5min, and remove the supernatant after centrifugation.
[0045] Step 2: Accurately draw 10mL of the subnatant into a 15mL graduated centrifuge tube, add 850mg of anhydrous magnesium sulfate, 100mg of PSA, and 150mg of C18, vortex for 1.5min, centrifuge at 5000r / min for 5min, and draw 1mL of the supernatant through a 0.2μm microporous filter Membrane, to obtain sample purification solution, to be determined by LC-MS / MS.
[0046] Step 3: Preparation of standard working solutions of sugar glufosinate and sulcotrione. Ac...
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Abstract
The invention discloses a quantitative determination method for residual quantity of quizalofop-p-tefuryl and sulcotrione in pig muscle and kidney tissue. The method comprises the following steps: preparing a quizalofop-p-tefuryl and sulcotrione standard solution and a sample extracting solution to be detected; extracting and purifying the sample; researching liquid phase and mass spectrum detection conditions; performing linear regression analysis and drawing a standard curve; representing the accuracy and the precision by the recovery rate and the variable coefficient respectively; and determining an actual sample and calculating a result. According to the method, discussion and optimization of the pretreatment technology and the determination method are carried out on target drug peaks, the method is high in accuracy, good in precision and sensitivity and simple, convenient and rapid in pretreatment operation, and powerful technical support is provided for guaranteeing food safety of people in China and promoting foreign export trade development.
Description
technical field [0001] The invention relates to a method for determining the residues of sugarglucoside and sulcotrione in pig muscle and kidney tissue. The involved matrix includes pig muscle and kidney tissue, and the LC-MS / MS detection method is used to measure sugar grass in the above two kinds of matrix. and the residual amount of sulcotrione, the method belongs to the technical field of determination of pesticide residues. Background technique [0002] With the rapid development of agricultural modernization, the types of herbicides are constantly updated. In order to achieve the expected harvest effect of crops, many crops choose to use multiple types of herbicides in large quantities during the planting process. Both sugar grass ester and sulcotrione are herbicides. The sugar glufosinate in this invention can be used to replace glyphosate, glufosinate and other organophosphorus herbicides that pollute the environment, and is widely used in the fields of dicotyledon...
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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/7266
Inventor 陈其勇王永芳刘旸王飞
Owner ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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