Quantitative determination method for residual quantity of quizalofop-p-tefuryl and sulcotrione in pig muscle and kidney tissue
A technology for the quantitative determination of sulcotrione, which is applied in the field of determination of pesticide residues, can solve the problems of no retrieval of glycyrrhizin and no detection method, etc.
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Embodiment 1
[0032] Example 1: Detection of glycoside and sulcotrione residues in pig kidney tissue:
[0033] Step 1: Take about 500g of pig kidney tissue, pulverize it evenly with a tissue grinder, put it into a clean container and seal it, and mark it. Weigh 2g of the sample, put it into a 50mL centrifuge tube, add 5mL of water, add 20mL of acidified acetonitrile, sonicate for 6min, add 2g of anhydrous sodium sulfate, vortex for 1min, centrifuge at 4500r / min for 5min, and remove the supernatant after centrifugation for later use.
[0034] Step 2: Accurately draw 10mL of the subnatant into a 15mL graduated centrifuge tube, add 850mg of anhydrous magnesium sulfate, 100mg of PSA, and 150mg of C18, vortex for 1.0min, centrifuge at 5000r / min for 5min, and draw 1mL of the supernatant through a 0.2μm microporous filter Membrane, to obtain sample purification solution, to be determined by LC-MS / MS.
[0035] Step 3: Preparation of standard working solutions of sugar glufosinate and sulcotrione. ...
Embodiment 2
[0043] Example 2: Detection of sugar glucoside and sulcotrione residues in pig muscle tissue:
[0044] Step 1: Take about 500g of pig muscle tissue, pulverize it evenly with a tissue grinder, put it into a clean container and seal it, and mark it. Weigh 2g of the sample, put it into a 50mL centrifuge tube, add 5mL of water, add 25mL of acidified acetonitrile, ultrasonicate for 8min, add 3g of anhydrous sodium sulfate, vortex for 1min, centrifuge at 4500r / min for 5min, and remove the supernatant after centrifugation.
[0045] Step 2: Accurately draw 10mL of the subnatant into a 15mL graduated centrifuge tube, add 850mg of anhydrous magnesium sulfate, 100mg of PSA, and 150mg of C18, vortex for 1.5min, centrifuge at 5000r / min for 5min, and draw 1mL of the supernatant through a 0.2μm microporous filter Membrane, to obtain sample purification solution, to be determined by LC-MS / MS.
[0046] Step 3: Preparation of standard working solutions of sugar glufosinate and sulcotrione. Ac...
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