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Method for the production of enzymes by a strain belonging to a filamentous fungus

A filamentous fungus and strain technology, applied in the directions of microorganism-based methods, methods of using microorganisms, biochemical equipment and methods, etc., can solve the problems of pH increase, disturbing the necessary adjustment of pH, laborious, etc., to achieve accurate size, increase usable The effect of volume

Pending Publication Date: 2021-07-30
INST FR DU PETROLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To name a few disadvantages: Resuspension of the foam as a liquid increases the pH considerably, greatly disturbing the necessary adjustment of the pH, and even causing undesired tipover from the growth step to the production step
However, this solution is laborious to implement due to the need to make these genetic modifications to each target strain

Method used

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  • Method for the production of enzymes by a strain belonging to a filamentous fungus
  • Method for the production of enzymes by a strain belonging to a filamentous fungus
  • Method for the production of enzymes by a strain belonging to a filamentous fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 was carried out starting with strain TR3002. This strain is described in the following publications: Ben Chaabane F, Jourdier E, Licht R, Cohen C and Monot F (2012) "Kinetic characterization of Trichoderma reesei CL847 TR3002: an engineered strain producing highly improved cellulolytic cocktail, Journal of Chemistry and Chemical Engineering6 ( 2), 109-117 and Ayrinhac C, Margeot A, Ferreira NL, Ben Chaabane F, Monot F, RavotG, Sonet J.-M and Fourage L (2011), "Improved saccharification of wheat straw forbiofuel production using an engineered secretome of Trichoderma reesei", Organic Process Research and Development 15 (1), 275-278.

[0077] - The growth phase was carried out at pH 4, 27°C with a glucose concentration of 15 g / L;

[0078] - The production phase was carried out at pH 4, 25°C with a lactose concentration of 220 g / L (equivalent to a specific fed-batch lactose flow rate of 45 mg per gram of biomass per hour).

Embodiment 2

[0079] Embodiment 2: (comparative example)

[0080] Example 2 was carried out starting with strain TR3002.

[0081] - The growth phase was carried out at a pH of 4.8 at 27°C with a glucose concentration of 15 g / L;

[0082] - The production phase was carried out at pH 4.8 at 25°C with a lactose concentration of 220 g / L (equivalent to a specific fed-batch lactose flow rate of 45 mg per gram of biomass per hour).

Embodiment 3

[0083] Embodiment 3: (comparative example)

[0084] Example 3 was carried out starting with strain TR3002.

[0085] - The growth phase was carried out at a pH of 5.5 at 27°C with a glucose concentration of 15 g / L;

[0086] - The production phase was carried out at pH 5.5 at 25°C with a lactose concentration of 220 g / L (equivalent to a specific fed-batch lactose flow rate of 45 mg per gram of biomass per hour).

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Abstract

The present invention concerns a method for the production of enzymes by a strain belonging to a filamentous fungus, the method comprising two steps: (a) a first step of growing the fungi, in the presence of at least one carbonaceous growth substrate, in a stirred and aerated batch bioreactor, at a pH less than or equal to 4.6; (b) a second step of producing enzymes, from the culture medium obtained in the first step (a), in the presence of at least one inductive carbonaceous substrate, at a pH less than or equal to 4.6.

Description

technical field [0001] The present invention relates to a process using filamentous fungi for the production of cellulase enzymes required, for example, for the enzymatic hydrolysis of lignocellulosic biomass in processes for the production of "second generation" (2G) sugar liquors. These sugar liquors can be used to produce other products via chemical or biochemical / fermentative pathways (eg alcohols such as ethanol biofuels, or butanol or other molecules such as solvents such as acetone and other bio-based molecules, etc.). Cellulases can also be used in other processes, especially in the chemical, paper or textile industries. [0002] As a concrete example of this implementation, the development of economically viable methods of producing second generation (2G) biofuels is the subject of much research. These biofuels are mainly made from woody substrates such as various types of wood (hardwood and softwood, miscanthus or SRC, which is an abbreviation for short-rotation bus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645C12P7/10C12N1/16C12N1/22C12N9/42C12N5/00C12R1/885C12P19/14C12N9/24C12P1/02
CPCC12N1/22C12N9/2437C12N2500/60C12P19/14C12P21/02Y02E50/10C12P21/00C12R2001/885C12N1/14C12N2500/05C12Y302/01004
Inventor F·本沙班C·科亨
Owner INST FR DU PETROLE
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