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Process for enzymolysis of sea cucumbers into multiple amino acids

A sea cucumber and process technology, applied in the field of enzymatic hydrolysis of sea cucumber, can solve the problems of low hydrolysis rate of sea cucumber protein and low purity of hydrolyzate, and achieve the effects of improving purity, simple treatment process, and low corrosion

Pending Publication Date: 2021-08-03
浙江泰利森药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the sea cucumber has not been subjected to previous impurity removal treatment, the purity of the sea cucumber enzymatic hydrolyzed solution is relatively low
At the same time, after sea cucumber beating, this method does not carry out proper heat treatment to the sea cucumber slurry to denature the sea cucumber protein to reduce the viscosity of the slurry. rate is also lower

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 The process of sea cucumber enzymatic hydrolysis into various amino acids comprises the following steps:

[0020] Take 100g sea cucumber dry sample and place it in 1000ml pure water to soak for 1 day; put the sea cucumber soaked in pure water into a volume fraction of 1% and soak it in 1mol / L sodium hydroxide for 1 day, stir once every 3 hours, Change the solution every 12 hours; wash the sea cucumber soaked in sodium hydroxide to neutrality, place the concentration of 1% by volume and soak it in 1.5mol / L hydrochloric acid for 1 day, stir once every 3 hours, Change the solution every 12 hours; wash the sea cucumber soaked in hydrochloric acid until the pH is 7, put it in a beater and break it into a sea cucumber slurry; put the sea cucumber slurry in a constant temperature water bath, and heat it at 90°C for 30 minutes for heat treatment; Cool the sea cucumber slurry after heat treatment to 50°C, fully stir the cooled sea cucumber slurry and pepsin in a mas...

Embodiment 2

[0021] Embodiment 2 The process of enzymolyzing sea cucumbers into various amino acids comprises the following steps:

[0022] Take 100g sea cucumber dry sample and place it in 1000ml pure water to soak for 2 days; put the sea cucumber soaked in pure water into a volume fraction of 1% and soak it in 1.5mol / L sodium hydroxide for 2 days, stir once every 3 hours, Change the solution every 12 hours; wash the sea cucumber soaked in sodium hydroxide to neutrality, place it in a concentration of 1% by volume and soak it in 2mol / L hydrochloric acid for 2 days, stir once every 3 hours, Change the solution every hour; wash the sea cucumber soaked in hydrochloric acid until the pH is 7.5, put it in a beater and break it into a sea cucumber slurry; put the sea cucumber slurry in a constant temperature water bath, and heat it at 92°C for 25 minutes for heat treatment; After the sea cucumber slurry was cooled to 45°C, the cooled sea cucumber slurry and A.S1398 neutral protease were fully s...

Embodiment 3

[0023] Embodiment 3 The process of enzymolyzing sea cucumbers into various amino acids comprises the following steps:

[0024] Take 100g dried sea cucumber samples and soak them in 1000ml pure water for 1 day; place the sea cucumbers soaked in pure water in a volume fraction of 1% and soak them in 1mol / L sodium hydroxide for 2 days, stir once every 3 hours, Change the solution every 12 hours; wash the sea cucumbers soaked in sodium hydroxide until neutral, put them in 2mol / L hydrochloric acid with a volume fraction of 1% and soak them for 2 days, stir once every 3 hours, Change the solution once; wash the sea cucumber soaked in hydrochloric acid until the pH is 8, put it in a beater and break it into a sea cucumber slurry; put the sea cucumber slurry in a constant temperature water bath, and heat it at 95°C for 20 minutes for heat treatment; The sea cucumber slurry was cooled to 45°C, and the cooled sea cucumber slurry and flavor protease were fully stirred evenly in a mass ra...

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Abstract

The invention discloses a process for enzymolysis of sea cucumbers into various amino acids, and relates to the technical field of enzymolysis of sea cucumbers. According to the method for effectively removing impurities on the surface of the sea cucumber, calcium, non-collagen substances and other impurities on the surface of the sea cucumber can be effectively removed through alkaline leaching and acid leaching treatment. The invention also provides a process for enzymolysis of sea cucumbers into various amino acids based on removal of surface impurities. By adopting the process disclosed by the invention, no other impurities are introduced, and calcium, non-collagen substances and other impurities on the surface of the sea cucumber can be effectively removed, so that the sea cucumber enzymatic hydrolysate with high purity and high hydrolysis rate is prepared. The process is simple in treatment process, easy to operate, low in equipment requirement and small in corrosion.

Description

[0001] This application is a divisional application of a Chinese patent application with an application date of 2015.03.26, an application number of 201510135348.6, and an invention titled "A Process for Enzymatically Hydrolyzing Sea Cucumbers into Various Amino Acids". The applicant is Zhejiang Tailisen Pharmaceutical Co., Ltd. . technical field [0002] The invention belongs to the technical field of enzymatic hydrolysis of sea cucumbers, and in particular relates to a process for enzymatic hydrolysis of sea cucumbers into various amino acids. Background technique [0003] Sea cucumbers are rich in protein and various trace elements, and are regarded as nutritionally efficient supplements. At present, people's processing and eating methods of sea cucumbers mainly include: cooking, raw food and making sea cucumber nutritional supplements. Cooking and eating will lead to the loss of a large amount of natural active ingredients in the cooking process; direct raw food will ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04
Inventor 崔生法韩风周中根孙雨良肖亚宝
Owner 浙江泰利森药业有限公司