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Lactobacillus johnsonii EU03 and application thereof

A technology of Lactobacillus johnsonii and Lactobacillus johnsonii, applied in the field of microbiology, can solve problems such as adverse reactions and increased blood concentration of creatine kinase, reduce inflammatory infiltration, improve myocardial injury, and improve serum enzymes The effect of indicators

Active Publication Date: 2021-08-06
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

β-blockers have been reported to have certain risks in intravenous application and adverse reactions such as fatigue, dizziness, depression and sexual dysfunction
Regulation of blood lipid statins has also been confirmed in recent years to reduce the occurrence of cardiovascular risk events, but it has been reported in the literature that the only serious adverse event caused by long-term statin therapy (that is, adverse reactions of statins) is myopathy (defined as muscle Pain or weakness with a large increase in serum creatine kinase concentration)
In summary, the current drugs for the treatment of MI all have a certain degree of adverse reactions. Therefore, the development of drugs that have no side effects and improve MI requires further exploration by researchers.

Method used

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  • Lactobacillus johnsonii EU03 and application thereof
  • Lactobacillus johnsonii EU03 and application thereof
  • Lactobacillus johnsonii EU03 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Lactobacillus johnsonii EU03 has antioxidant activity and improves myocardial injury indicators in rats after MI

[0041] As shown in Table 1, the experiment was divided into three groups, the control group (4 weeks), the model group (4 weeks) and the bacteria-administered group (4 weeks), and the serums of the three groups of experimental rats were used as test samples.

[0042] Model group (4 weeks): Rats were fed adaptively for one week, fasted for 12 hours before operation, and had free access to water. Before modeling, the rats were weighed, and the rats were anesthetized by intraperitoneal injection of 0.6 mL / 100 g body weight of 5% chloral hydrate. Prepare the chest skin of the anesthetized rat, fix it in the supine position, disinfect the chest skin with alcohol or povidone iodine, cut along the left fourth intercostal space (or the place where the heart beat is most obvious), and separate the subcutaneous tissue layer by layer with hemostatic force...

Embodiment 2

[0062] Embodiment 2: Lactobacillus johnsonii EU03 improves heart function in MI rats

[0063] After 4 weeks of administration, the cardiac function of the rats was tested. Rats were anesthetized by intraperitoneal injection of 5% chloral hydrate and then depilated with depilatory cream. Vevo2100 ultra-high resolution small animal ultrasound real-time imager (Canada) is ready for use after starting up. Fix the probe MS-250, adjust the height of the probe by the lifting table, and adjust the direction of the probe by rotating it. Ultrasound was performed with isoflurane anesthesia (complete anesthesia: 1% oxygen + 5% isoflurane in anesthesia box; continuous anesthesia: 1% oxygen + 2% isoflurane). Use adhesive tape to fix the abdomen of the rat upward on the operating table, set the temperature at 37°C; apply a layer of ultrasonic coupling agent evenly on the chest of the rat, tilt the operating table 30-45° downward to the right, and fine-tune the operating table through X an...

Embodiment 3

[0067] Embodiment 3: Lactobacillus johnsonii EU03 can restore normal myocardial morphology in MI rats

[0068] After MI, cardiac dysfunction occurs, resulting in ischemic necrosis of cardiomyocytes, destruction of cardiac morphology and collagen fibers. In order to maintain the integrity of the structure, the damaged heart pathologically improves collagen metabolism and forms the pathological morphological characteristics of the coexistence of collagen and myocardium. In this experiment, hematoxylin-eosin staining (hematoxylin-eosin staining, HE) and Masson staining were used to compare the degree of myocardial lesion, so as to observe the effect of Lactobacillus johnsonii EU03 on myocardial morphology of MI rats.

[0069] Take heart tissue fixed in 10% formaldehyde solution for at least 48 hours, change the knife to cut out a tissue with a thickness of about 2-3 mm, and process it in the following order: 75% ethanol for 1 h→95% ethanol overnight→100% ethanol I for 1 h→100% ...

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Abstract

The invention belongs to the technical field of microorganisms, and discloses a lactobacillus johnsonii EU03 (Lactobacillus johnsonii EU03) and an application thereof. The lactobacillus johnsonii has antioxidant activity, and can be used for improving serum enzymology indexes of a myocardial infarction (MI) rat and repairing myocardial injury; a heart function index of the MI rat can be improved; and the damaged heart recovers the normal myocardial morphological effect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Lactobacillus johnsonii EU03 (Lactobacillus johnsonii EU03) and its application, especially a Lactobacillus johnsonii EU03 capable of improving myocardial injury, heart function and myocardial morphology and its application. Background technique [0002] Cardiovascular disease and related diseases, such as hypercholesterolemia, hypertension, obesity, diabetes, are the leading causes of death worldwide and cause economic loss. Myocardial infarction (MI) has received widespread attention as an important cause of cardiovascular death. Currently, there are various problems in the development of drugs for the treatment of MI. In clinical practice, glucocorticoids were used to treat MI in the early stage, and then it was found that such drugs would increase the risk of cardiac rupture in MI patients [4]. Non-steroidal anti-inflammatory drugs have also been widely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P9/00A23L29/00A23L33/135A23L11/00A23L19/00A23C9/123C12R1/225
CPCC12N1/20A61K35/747A61P9/00A23L29/065A23L33/135A23L19/00A23C9/1234A23V2002/00A23V2400/151A23V2200/30
Inventor 王小莹高秀梅赵鑫钟鑫勤魏兴
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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