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A kind of pharmaceutical preparation and method for resisting evening primrose mosaic virus

A leaf virus and preparation technology, which is applied in the field of medicinal preparations against nocturnal mosaic virus, can solve the problems of high production cost, hidden dangers of human, animal and ecological environment safety, and achieves improved control, alleviation of growth inhibition, and reduction of accumulation. Effect

Active Publication Date: 2021-12-31
广东省科学院南繁种业研究所
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0004] Once a susceptible plant is found in the field, the method of uprooting the diseased plant is mainly adopted to reduce the further expansion of the disease for the susceptible seedling; for the susceptible plant that has already hung a large number of fruits, considering the cost problem, usually by spraying various energy Pesticides or biological agents that inactivate viruses or induce plant immunity, and try to inhibit the spread of virus diseases to new vines and leaves, but this method has a high production cost and has great safety risks for humans, animals and the ecological environment Hidden danger

Method used

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  • A kind of pharmaceutical preparation and method for resisting evening primrose mosaic virus
  • A kind of pharmaceutical preparation and method for resisting evening primrose mosaic virus
  • A kind of pharmaceutical preparation and method for resisting evening primrose mosaic virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Effect of Rls on TeMV virus

[0037] Experimental materials: Rls (Sigma, purity ≥ 90%), golden passion fruit seedlings (the seedlings are cultivated from the golden passion fruit seeds preserved by our laboratory, and the fruits were purchased from the passion fruit digital orchard in Dapu County, Meizhou City, Guangdong Province, After harvesting the fruit seeds, they were naturally air-dried and stored in our laboratory for a series of basic scientific research), and were confirmed to be the source of the TeMV virus after identification (through the sequencing analysis and comparison of the PCR product of the virus sample: PCR The obtained target fragment is highly similar to the TeMV sequence of the known database (the GenBank accession number of the reference sequence is KJ789129.1) (specifically as figure 1 As shown, the similarity is 98.43%), confirming that the sample is TeMV virus).

[0038] Through the preliminary experiment, it was found that the in...

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Abstract

The present invention relates to a medicinal preparation and method for resisting the Echinacea mosaic virus, the active components of which include rhamnolipid. The inventors found through experiments that rhamnolipid can effectively reduce the accumulation of TeMV on plants, thereby effectively alleviating the mosaic disease of passion fruit caused by TeMV. In addition, through the study of physiological and biochemical mechanisms, it was found that Rls can effectively alleviate the growth inhibition of passion fruit plants caused by virus infection, and can significantly improve the resistance and defense ability of passion fruit plants to TeMV. It indicates that it has potential in improving plant resistance to viruses and the prevention and control of plant virus diseases, and has broad application prospects in future agricultural production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a pharmaceutical preparation and method for resisting evening primrose mosaic virus. Background technique [0002] As a nutritious fruit, passion fruit has developed rapidly in the southern part of my country in recent years. It has gradually become an important channel for farmers in Guangxi, Guangdong, Fujian and Hainan to increase their income. Passion fruit cultivation has become a key promotion project for rural poverty alleviation . However, in recent years, due to improper planting management, passion fruit has been continuously harmed by various diseases and insect pests, among which viral diseases have become one of the serious constraints on the production of passion fruit, and especially mosaic virus diseases have seriously affected plant growth and even Fruit yield and quality. Therefore, starting from the needs of national economic development, relying on the stable dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01N63/20A01P1/00
CPCA01N43/16A01N63/20
Inventor 陈丽娟安玉兴孙东磊张兴兴邵丹青卢颖林
Owner 广东省科学院南繁种业研究所
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