Epsilon-polylysine-natamycin synergistic production method based on interactive recycling of fermentation waste fungus residues

A technology of natamycin and polylysine, which is applied in the field of industrial biology, can solve the problems of pollution, waste of the environment, and inability to be further utilized, and achieve the effect of increasing fermentation yield and great application value

Active Publication Date: 2021-09-03
HUAIBEI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The precipitate (fermentation residue) is discarded after the product has been extracted and cannot be further utilized
ε-polylysine is mainly synthesized by Streptomyces parvus through liquid fermentation. Its p...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: Resource extraction of natamycin and ε-polylysine fermentation waste bacteria residue

[0058] The ε-polylysine fermentation broth obtained by fed-batch fermentation for 168 hours was subjected to solid-liquid separation, and the bacterial residue was washed three times with deionized water, and the washing liquid was incorporated into the fermentation supernatant for extraction of ε-polylysine Acid, wet bacterial cells were weighed, then soaked and ground with a final concentration of 75% ethanol aqueous solution, let it stand overnight, and the ground liquid was centrifuged at 3000-4500rpm for 10-15min, and the supernatant was placed in a rotary evaporator to distill and recover the solvent. The concentrated solution was dried in an oven at 60°C, and the extract was redissolved with 1 g of wet bacterial residue corresponding to 3 mL of deionized water to obtain an extract of ε-polylysine fermentation waste bacterial residue;

[0059] Centrifuge the natamyci...

Embodiment 2

[0060] Example 2: Optimizing the Addition Conditions of ε-Polylysine Fermentation Waste Bacteria Residue Extract by Natamycin Erlenmeyer Flask Fermentation

[0061] In a 250mL Erlenmeyer flask, configure the liquid medium according to the formula of natamycin fermentation medium, seal it with 8 layers of gauze, and sterilize it in a high-temperature sterilization pot at 115°C for 20min; inoculate the mature fermented seeds with 8% inoculum The above fermentation medium was cultured in a constant temperature shaking incubator at 29° C. at 220 rpm for 3 days, and the concentration of natamycin in the fermentation broth was measured after the end.

[0062] Add the extract with a final concentration of 5gε-polylysine fermentation wet residue / L culture medium at 0, 12, 18, 24, 30, 36, 42, and 48 hours of fermentation respectively, and investigate the final natamycin production. The results are as follows : Control (deionized water instead of extract) 0.6g / L, 0h 0.7g / L, 12h 0.7g / L, ...

Embodiment 3

[0064] Example 3: Fed-batch fermentation of natamycin 5L fermenter with addition of ε-polylysine fermentation waste slag extract

[0065]The 5L fermenter adopts 2 sets of 6 straight-bladed turbine impellers, the liquid volume in the fermenter is set to 3L, the natamycin fermentation medium is selected, the ventilation volume is 1vvm, and the speed is set at 200-800rpm. By increasing the speed Dissolved oxygen in the entire fermentation process is controlled above 30% by means of a method, and the pH of the fermentation broth is adjusted to pH 7.0 with sodium hydroxide. The fermenter was sterilized by off-site sterilization at 121°C for 20 minutes. After the culture medium was cooled to 29°C, 8% mature seed liquid was inoculated for fermentation. After 24 hours of fermentation, exogenous extracts with a final concentration of 5gε-polylysine fermentation wet residue / L medium were added, and the fermentation was continued thereafter. At 48 hours, 60% glucose was started to flow ...

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PUM

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Abstract

The invention discloses an epsilon-polylysine-natamycin synergistic production method based on interactive recycling of fermentation waste fungus residues. The production method comprises the following steps of extracting an inducer by utilizing natamycin fermentation waste thalli, and adding the inducer in epsilon-polylysine fermentation to induce mass synthesis of epsilon-polylysine; and meanwhile, extracting waste epsilon-polylysine thalli, and adding the extract into natamycin fermentation to induce synthesis of a large amount of natamycin. The method is beneficial to resource utilization of the waste thalli of the natamycin and the epsilon-polylysine, has the effect of promoting synthesis of the two products, and has important practical significance in industrial production of the natamycin and the epsilon-polylysine; and particularly, the method has a relatively great application value in enterprise production with production lines of three biological preservatives including natamycin, nisin and epsilon-polylysine.

Description

technical field [0001] The invention relates to an epsilon-polylysine-natamycin synergistic production method based on the interactive reuse of fermentation waste bacteria residue, which belongs to the field of industrial biotechnology. Background technique [0002] The existing microbial preservatives mainly include natamycin, nisin, and ε-polylysine. In order to improve product coverage and enhance integration advantages, many manufacturers have built the above three biological preservatives at the same time production line. Two of these three biopreservatives (natamycin and ε-polylysine) are synthesized by Streptomyces, of which natamycin is a polyene macrolide antifungal agent, and its Strong inhibitory effect on the growth of mold and yeast; ε-polylysine is a homotype amino acid cationic polymer, which has a significant inhibitory effect on the growth of Gram-positive and negative bacteria, yeast and mold. Both of these two types of substances have high safety and are...

Claims

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Application Information

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IPC IPC(8): C12P13/02C12P19/62C12N1/14C12R1/465
CPCC12P13/02C12P19/626C12N1/14
Inventor 曾昕李峰曾化伟信丙越徐大勇张标李珊珊
Owner HUAIBEI NORMAL UNIVERSITY
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