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Culture medium for inhibiting endophyte and material browning in tissue culture of date palm and application

A technology of endophytic bacteria and date palms, applied in the field of plant biology, to reduce damage, reduce tissue culture costs, and prevent spore germination

Active Publication Date: 2021-09-07
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no use and record of this technology in the field of date palm tissue culture both at home and abroad at present.

Method used

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  • Culture medium for inhibiting endophyte and material browning in tissue culture of date palm and application
  • Culture medium for inhibiting endophyte and material browning in tissue culture of date palm and application
  • Culture medium for inhibiting endophyte and material browning in tissue culture of date palm and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Medium for inhibiting endophytic bacteria and material browning in date palm tissue culture, including reagent A, reagent B and reagent C;

[0065] The reagent A is a 1 / 2MS salt solution containing Shannong No. 1 type I fungicide (final concentration 1% wt);

[0066]The reagent B contains Shannong No. 1 type III bactericide (final concentration 0.1%wt), plant gel (final concentration 3.5g / L), sucrose (final concentration 30g / L), glutamine (final concentration 100mg / L) L), pyridoxine hydrochloride (final concentration 1mg / L), niacin (final concentration 0.5mg / L), 6-benzylaminoadenine (final concentration 0.2mg / L), isopentenyl adenine (final concentration 0.1mg / L), 2,4-dichlorophenoxyacetic acid (final concentration 0.02mg / L), thiadizuron (final concentration 0.02mg / L), naphthaleneacetic acid (final concentration 0.08mg / L), polyvinylpyrrolidone MS salt solution of (final concentration 0.5g / L), ethanesulfonic acid monohydrate (final concentration 1g / L) and activated carbo...

Embodiment 2

[0082] Medium for inhibiting endophytic bacteria and material browning in date palm tissue culture, including reagent A, reagent B and reagent C;

[0083] The reagent A is a 1 / 2MS salt solution containing Shannong No. 1 type I fungicide (final concentration 4.5%wt);

[0084] The reagent B contains Shannong No. 1 type III bactericide (final concentration 0.12%wt), plant gel (final concentration 3.5g / L), sucrose (final concentration 30g / L), glutamine (final concentration 100mg / L) L), pyridoxine hydrochloride (final concentration 1mg / L), niacin (final concentration 0.5mg / L), 6-benzylaminoadenine (final concentration 0.5mg / L), isopentenyl adenine (final concentration 0.2mg / L), 2,4-dichlorophenoxyacetic acid (final concentration 0.05mg / L), thiadizuron (final concentration 0.05mg / L), naphthaleneacetic acid (final concentration 0.1mg / L), polyvinylpyrrolidone MS salt solution of (final concentration 0.5g / L), ethanesulfonic acid monohydrate (final concentration 1g / L) and activated car...

Embodiment 3

[0100] Medium for inhibiting endophytic bacteria and material browning in date palm tissue culture, including reagent A, reagent B and reagent C;

[0101] The reagent A is a 1 / 2MS salt solution containing Shannong No. 1 type I fungicide (final concentration 5% wt);

[0102]The reagent B contains Shannong No. 1 type III fungicide (final concentration 0.2%wt), plant gel (final concentration 3.5g / L), sucrose (final concentration 30g / L), glutamine (final concentration 100mg / L) L), pyridoxine hydrochloride (final concentration 1mg / L), niacin (final concentration 0.5mg / L), 6-benzylaminoadenine (final concentration 0.8mg / L), isopentenyl adenine (final concentration 0.5mg / L), 2,4-dichlorophenoxyacetic acid (final concentration 0.08mg / L), thiadizuron (final concentration 0.08mg / L), naphthaleneacetic acid (final concentration 0.12mg / L), polyvinylpyrrolidone MS salt solution of (final concentration 1g / L), ethanesulfonic acid monowater (final concentration 1g / L) and activated carbon (fin...

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Abstract

The invention discloses a culture medium for inhibiting endophyte and material browning in tissue culture of date palm. The culture medium comprises a reagent A, a reagent B and a reagent C; the reagent A is a 1 / 2 MS salt solution containing a Shannong No.1 I-type bactericide; the reagent B is an MS salt solution containing a Shannong No.1 III-type bactericide, plant gel, cane sugar, glutamine, pyridoxine hydrochloride, nicotinic acid, 6-benzylamino adenine, isopentenyl adenine, 2, 4-dichlorphenoxyacetic acid, thidiazuron, naphthylacetic acid, polyvinylpyrrolidone, ethanesulfonic acid monohydrate and activated carbon; the reagent C is an MS salt solution containing plant gel, cane sugar, glutamine, pyridoxine hydrochloride, nicotinic acid, inositol, 6-benzylamino adenine, isopentenyl adenine, 2,4-dichlorphenoxyacetic acid, naphthylacetic acid, polyvinylpyrrolidone, ethanesulfonic acid monohydrate and activated carbon. According to the culture medium, breeding of endophytes can be inhibited in the culture process, the pollution rate of the endophytes is controlled to be 9% or below, and the browning rate of the explants is controlled to be 13% or below.

Description

technical field [0001] The invention relates to the field of plant biotechnology, and more specifically relates to a culture medium for inhibiting endophytic bacteria and browning of materials during tissue culture of date palms, a preparation method and a use method. Background technique [0002] Date palm is a perennial tropical palm plant with dioecious plants and is an important woody food crop in Arab countries. Date palms can use seeds to reproduce offspring, but because it is a dioecious plant and is in a highly heterozygous state, this method of reproduction will lose the target traits of the parents in production, and cannot guarantee the excellent characteristics and fruit of the mother plant. feature. [0003] At present, in the world, branching of tiller seedlings and tissue culture are the main methods for the reproduction of date palm seedlings. During the growth cycle of each date palm seedling, the number of tiller seedlings is 15-25, which is severely limit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/005
Inventor 徐中亮张宁王义符海泉王富有
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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