A kind of preparation method of small molecule sodium hyaluronate

A technology of sodium hyaluronate and small molecules, applied in the field of preparation of small molecule sodium hyaluronate, can solve problems such as easy yellowing, and achieve the effects of promoting degradation, reducing molecular weight and increasing solubility

Active Publication Date: 2022-04-08
上海加新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to improve the problem that macromolecular sodium hyaluronate is easily turned yellow when degraded into small molecular sodium hyaluronate, this application provides a preparation method of small molecular sodium hyaluronate

Method used

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  • A kind of preparation method of small molecule sodium hyaluronate
  • A kind of preparation method of small molecule sodium hyaluronate
  • A kind of preparation method of small molecule sodium hyaluronate

Examples

Experimental program
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Embodiment 1

[0044] A preparation method of small molecule sodium hyaluronate, characterized in that it comprises the following steps:

[0045] S1: Add 5L of organic solvent to the ultrasonic high-pressure multifunctional reaction kettle, and dissolve 0.5kg of sodium hyaluronate solid with a molecular weight of 1.2MDa in 5L of organic solvent at a stirring speed of 1000r / min, and stir and mix for 0.5h ; At 40°C, add 0.04L of acetic acid and 0.02L of hydrogen peroxide dropwise to the ultrasonic high-pressure multifunctional reaction kettle in sequence. After the ultrasonic frequency is 15KHz, after ultrasonic stirring for 15min, the stirring speed is 1000r / min. , stirred for 5h to obtain a suspension;

[0046] S2: Under the condition that the drum speed is 1200r / min, the suspension is collected by a centrifuge; Liquid, control the temperature of the ultrasonic high-pressure multifunctional reactor to be -5-0 °C, adjust the pH value of the mixed solution to 9 with 1mol / L sodium hydroxide so...

Embodiment 2

[0051] A method for preparing small-molecule sodium hyaluronate, characterized in that, the difference from Example 1 is that in the preparation process S1 of small-molecule sodium hyaluronate, the organic solvent is 90% methanol aqueous solution, and the mixture is stirred for 6.5 hours; In S2, adjust the pH value of the mixed solution B to 10 with 1mol / L sodium hydroxide solution.

Embodiment 3

[0053] A method for preparing small-molecule sodium hyaluronate, which is characterized in that, the difference from Example 1 is that in the preparation process S1 of small-molecule sodium hyaluronate, the organic solvent is 90% methanol aqueous solution, stirred for 8 hours; S2 , adjust the pH of the mixture B to 11 with 1mol / L sodium hydroxide solution.

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Abstract

The application relates to the technical field of sodium hyaluronate production, more specifically, it relates to a preparation method of small molecule sodium hyaluronate. The preparation method of small molecule sodium hyaluronate comprises the following steps: dissolving sodium hyaluronate solid in an organic solvent with a volume concentration of 80-90%, sequentially adding acetic acid and hydrogen peroxide dropwise to the organic solvent, ultrasonication, and stirring React for 5-8h to obtain a suspension; centrifuge the suspension, collect the precipitate, dissolve the precipitate with a sodium chloride solution to obtain a mixed solution, adjust the pH of the mixed solution to 9-11, filter, collect the supernatant, and separate the upper layer The supernatant was precipitated, dehydrated and centrifuged, and dried in vacuum to obtain the target product. The preparation method of small molecule sodium hyaluronate of the present application can be used to degrade macromolecule sodium hyaluronate to obtain small molecule sodium hyaluronate without affecting the color of small molecule sodium hyaluronate.

Description

technical field [0001] The application relates to the technical field of sodium hyaluronate production, more specifically, it relates to a preparation method of small molecule sodium hyaluronate. Background technique [0002] Hyaluronic acid is a linear mucopolysaccharide formed by glucuronic acid and N-acetylglucosamine alternately linked by glycoside chains, and its molecular weight is tens of thousands to millions. Under physiological conditions, the hydroxyl group on the uronic acid group in the hyaluronic acid molecule is in a free state. When combined with sodium ions, the polysaccharide is in the form of sodium salt, which is called sodium hyaluronate. [0003] Low molecular weight sodium hyaluronate is the use of physical, chemical or biological methods to degrade macromolecular hyaluronic acid into sodium hyaluronate with relatively low molecular weight. Low-molecular-weight hyaluronic acid can penetrate into the dermis, slightly dilate capillaries, increase blood ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/0072
Inventor 刘英王华倩王红娟蔡建平
Owner 上海加新生物科技有限公司
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