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Fluorescence sensing system and preparation method and application thereof

A technology of fluorescence sensing and nano-fluorescence, which is applied in the field of fluorescence sensing system and its preparation, can solve the problems of complex operation, expensive equipment, long time, etc.

Active Publication Date: 2021-09-10
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In recent years, the detection methods of patulin mainly include gas phase/liquid chromatography, gas phase/liquid chromatography tandem mass spectrometry, enzyme-linked immunoassay and thin layer chromatography, etc. These detection methods have high sensitivity and a large amoun

Method used

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  • Fluorescence sensing system and preparation method and application thereof
  • Fluorescence sensing system and preparation method and application thereof
  • Fluorescence sensing system and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0045] Sulfhydryl-modified nano-fluorescent liposomes (CM6@Lip-SH) coated with coumarin 6 and amino-functionalized gold-coated ferric oxide nanoparticles (NH 2 -Au@Fe 3 o 4 ) preparation:

[0046] 1. The preparation of sulfhydryl-modified nano-fluorescent liposomes (CM6@Lip-SH) coated with coumarin 6, the specific steps are as follows:

[0047] (1) Dissolve 20 mg of cholesterol, 100 mg of soybean lecithin, 5 mg of DSPE-PEG-SH, and 0.1 mg of coumarin 6 in 15 mL of dichloromethane, and sonicate until uniformly dispersed. Transfer the dispersion to a round-bottomed flask and place it in a rotary evaporator to completely evaporate the solvent to form a thin film attached to the wall of the bottle. Add 2 mL of 0.01mol L to the round-bottomed flask -1 PBS buffer solution (pH=7.4) and 50μL TCEP (10mmol L -1 ) solution, continued to hydrate for 1 hour, and finally obtained a green gel-like liquid.

[0048] (2) Ultrasound the gel-like liquid obtained in (1) for 3 minutes under the...

Embodiment 2

[0059] The patulin fluorescent sensing system prepared in Example 1 measures patulin in samples

[0060] All fluorescence measurements were performed on a F-7000 spectrophotometer (Hitachi, Japan) with an excitation wavelength of 455 nm and an emission wavelength of 505 nm. The slit width for excitation and emission is 10 nm. Scanning speed is 1200nm min -1 , the response time is 0.5s. Photomultiplier tube (PMT) voltage is 400V.

[0061] All fluorescence measurements were carried out on a F-7000 spectrophotometer (Hitachi, Japan), the excitation wavelength was 455nm when performing experiments, and the emission wavelength was 505nm when collecting fluorescence intensity signals; the slit width for excitation and emission was 10nm; The speed is 1200nm min -1 , the response time is 0.5s; the photomultiplier tube (PMT) voltage is 400V;

[0062] Specific detection steps:

[0063] (1) Add 200 μL of sulfhydryl-modified nano-fluorescent liposome dispersion coated with coumarin ...

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Abstract

The invention provides a fluorescence sensing system. The fluorescence sensing system is composed of a coumarin 6 coated sulfydryl modified nano fluorescent liposome CM6 coated Lip-SH dispersion liquid and an amino functionalized gold coated ferroferric oxide nano particle NH2-Au coated Fe3O4 dispersion liquid. The fluorescence sensing system provided by the invention can be used for rapidly detecting patulin, and is good in selectivity, high in recovery rate, small in background interference and good in reproducibility; and the method does not need to depend on expensive large instruments, is simple in pretreatment, achieves rapid detection while guaranteeing the result accuracy, greatly saves the cost, and improves the detection efficiency.

Description

technical field [0001] The invention relates to the technical field of food detection and analysis, in particular to a fluorescent sensing system and its preparation method and application. Background technique [0002] Patulin (PAT) is a highly dangerous mycotoxin produced by Aspergillus, Penicillium and Filamentosa species, which is often found in fruits, especially in apples, grapes and their derivatives middle. Acute and chronic ingestion of patulin can cause damage to the respiratory and urinary systems, nerve paralysis, pulmonary edema, and renal failure. Due to the high toxicity and widespread presence of patulin, the United States, the European Union, and China have set a maximum limit of 50 ng mL in apple juice -1 . [0003] In recent years, the detection methods of patulin mainly include gas phase / liquid chromatography, gas phase / liquid chromatography tandem mass spectrometry, enzyme-linked immunoassay and thin layer chromatography, etc. These detection methods ...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/06
CPCG01N21/64G01N21/6428C09K11/06C09K11/025G01N2021/6417C09K2211/1088
Inventor 韩铮聂冬霞赵志辉郭大凯黄晴雯
Owner SHANGHAI ACAD OF AGRI SCI
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