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A strain of animal bifidobacterium nsy0201 and its application in the preparation of health products or medicines for enhancing body immunity

A NSY0201, animal bifidobacteria technology, applied in the field of microorganisms, can solve problems such as slow effect, achieve the effect of safe source, enhance immunity, enhance immune function and disease resistance

Active Publication Date: 2022-06-24
青岛诺森生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, dietary conditioning, enhanced exercise, zinc supplementation, etc. are often used to enhance immunity, but the effects of these are relatively slow

Method used

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  • A strain of animal bifidobacterium nsy0201 and its application in the preparation of health products or medicines for enhancing body immunity
  • A strain of animal bifidobacterium nsy0201 and its application in the preparation of health products or medicines for enhancing body immunity
  • A strain of animal bifidobacterium nsy0201 and its application in the preparation of health products or medicines for enhancing body immunity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Isolation and identification of Bifidobacterium animalis NSY0201

[0025] 1. Isolation and screening of strains

[0026] Take natural fermented cheese from Xilingol League, Inner Mongolia as a sample. Take 3 samples and add them to 10% skim milk medium according to the dosage of 1%, incubate at 37°C, and perform gradient dilution after curdling, and gradually dilute to 10%. -8 gradient. Choose 3 suitable dilutions, take 1 mL of each in the modified MRS solid medium, culture them anaerobically at 37°C for 48 hours, observe the colony morphology, select single colonies with different shapes and streak them on the modified MRS solid medium again, 37 Anaerobic culture at ℃ for 48h, repeated streak separation for 3 generations, to obtain purified strains, Gram staining and microscopic observation were carried out to select the strains that were Gram-positive, spherical, rod-shaped or multi-shaped under microscope observation. Six strains meeting the requirement...

Embodiment 2

[0036] Example 2: Physiological and biochemical properties of Bifidobacterium animalis NSY0201

[0037] After the preserved Bifidobacterium animalis NSY0201 was activated, it was inoculated into the modified MRS medium with an inoculum of 3%, cultivated at 37 °C for 16 h, and continuously cultivated for 3 generations to expand the culture. The obtained fermentation broth ( image 3 ) were used to conduct experiments on various physiological and biochemical characteristics. Among them, the formula of the improved MRS medium is: 18 grams of glucose, 10 grams of peptone, 7 grams of beef extract powder, 4 grams of yeast extract powder, 2 grams of diammonium hydrogen citrate, 5 grams of sodium acetate, 2 grams of dipotassium hydrogen phosphate, Magnesium sulfate 0.58g, manganese sulfate 0.25g, Tween-80 1.0ml, L-cysteine ​​hydrochloride 0.5g, distilled water 1000ml, pH=6.8±0.5.

[0038] 1. Routine physiological and biochemical experiments

[0039] The fermentation broth was used f...

Embodiment 3

[0061] Example 3: Preparation of Bifidobacterium animalis NSY0201 freeze-dried powder

[0062] The preserved Bifidobacterium animalis NSY0201 was streaked on the modified MRS solid plate, cultured and activated at 37 °C, and then a single colony was picked and inoculated into the modified MRS liquid medium, cultivated at 37 °C for 16 h, and then regenerated with 3% of the inoculum. Inoculated into the modified MRS medium, cultured at 37 °C for 16 h, and then continuously cultured for 2 generations to expand the culture; the obtained fermentation broth was centrifuged at 4 °C, 12000 r / min for 10 min, the supernatant was discarded, the bacterial slurry was collected, and ultrapure water was used. After washing 3 times, mix the bacterial paste and freeze-drying protective agent (the formula is 30g trehalose, 10g skim milk powder, 7g inulin, 0.5g isomaltooligosaccharide, 0.2g glycine, 1g Tween 80, 1000mL pure water) to After mixing in a mass ratio of 1:1.7, and fully emulsified, t...

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Abstract

The invention discloses a strain of animal bifidobacteria NSY0201 and its application in preparing health products or medicines for enhancing body immunity. The classification of the animal Bifidobacterium NSY0201 is named Animal Bifidobacterium Bifidobacterium animalis , preserved in China Center for Type Culture Collection, the preservation number is CTCC M 2021629, its nucleotide sequence is shown in SEQ ID No.1, and has the function of high-yield extracellular polysaccharide. The animal Bifidobacterium NSY0201 and its freeze-dried powder can enhance the body's immune function by inhibiting DNFB-induced DTH, increasing the serum hemolysin level, phagocytosis index and NK cell activity, and accelerating the proliferation of lymphocytes in mice. and disease resistance, and it is safe to use, can be used to prepare health products or medicines, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Bifidobacterium animalis NSY0201 and its application in preparing health-care products or medicines for enhancing body immunity. Background technique [0002] Immunity is the body's own defense mechanism, and is the ability of the body to recognize and eliminate any foreign intrusion (viruses, bacteria, etc.) . Humans have been able to survive in dangerous environments for millions of years, mainly relying on their own resistance. [0003] A weakened immune system is prone to infection or cancer. A weak immune system in children can lead to poor physical and mental development, some of which are prone to major illnesses, and can cause recurring health problems. Therefore, modern people pay more and more attention to the improvement of their own immunity. At present, dietary conditioning, exercise, and zinc supplementation are often used to enhanc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04A23L33/135A61K35/745A61P37/04C12R1/01
CPCC12N1/20C12N1/04A23L33/135A61K35/745A61P37/04A23V2002/00A23V2400/515A23V2200/324
Inventor 张明俊马超孙海燕刘翠玲赵志强
Owner 青岛诺森生物技术有限责任公司
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