Non-enzyme glucose electrochemical sensor and preparation method thereof

An electrochemical and glucose technology, applied in the field of biochemical sensors, can solve the problem of low catalytic activity of transition metal sulfides, achieve good anti-interference ability and stability, mild preparation conditions, and simple and fast preparation methods

Pending Publication Date: 2021-09-28
HUBEI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The catalytic activity of a single transition metal sulfide is not high, therefore, we developed a copper-cobalt-nickel complex sulfide enzyme-free glucose sensor

Method used

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  • Non-enzyme glucose electrochemical sensor and preparation method thereof
  • Non-enzyme glucose electrochemical sensor and preparation method thereof
  • Non-enzyme glucose electrochemical sensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Cut the copper foam into 0.8×1.5 cm 2 Sonicate in acetone and ethanol at 100kHz for 10min each.

[0034] (2) Prepare 0.5 M mixed solution of cobalt sulfate and nickel sulfate (the molar ratio of cobalt to nickel is 1:1) A, and then prepare 0.5 M sodium sulfide solution. As a comparison, 0.5 M cobalt sulfate solution B and 0.5 M nickel sulfate solution C were prepared separately.

[0035] (3) Immerse foamed copper in the above solution A for 1 min, place it upright on the filter paper with tweezers for 15 s, then immerse it in the above sodium sulfide solution for 1 min, rinse with water to obtain a copper-cobalt-nickel composite sulfide electrode. As a comparison, another three pieces of foamed copper were taken, one piece was directly immersed in sodium sulfide solution for 1 min, and the copper sulfide electrode was obtained after washing with water; the other two pieces were respectively immersed in B and C solutions for 1 min, and they were placed upright on t...

Embodiment 2

[0039] (1) Cut the copper foam into 0.8×1.5 cm 2 The squares were sonicated in acetone and ethanol at 100 kHz for 10 min each.

[0040] (2) Prepare 0.5 M cobalt sulfate and nickel sulfate mixed solution (the molar ratio of cobalt to nickel is 1:1), and prepare 0.5 M sodium sulfide solution.

[0041] (3) Immerse the foamed copper in the above cobalt-nickel mixed solution for 1 min, place it upright on the filter paper for 15 s with tweezers, then immerse it in the above sodium sulfide solution for 1 min, and rinse it with water to obtain a copper-cobalt-nickel composite sulfide electrode.

[0042] (4) Dry the copper-cobalt-nickel composite sulfide electrode obtained in step (3) at 60 °C for 3 h.

[0043] (5) The copper-cobalt-nickel composite sulfide electrode obtained in step (4) was used as the working electrode, and a three-electrode system was formed with the counter electrode (platinum wire electrode) and the reference electrode (Ag / AgCl / 3M KCl). Hua CHI660C electrochem...

Embodiment 3

[0048] The copper-cobalt-nickel trimetallic sulfide enzyme-free glucose sensor constructed above was applied to the anti-interference performance test. The specific steps and results were as follows: 0.1 mM glucose, 0.01 mM ascorbic acid (AA), 0.01 mM ascorbic acid (AA), 0.01 mM dopamine (DA), 0.01 mM uric acid (UA), 0.1 mM glucose, and time-current curves were tested. Such as Figure 6 As shown, the copper-cobalt-nickel enzyme-free glucose sensor constructed by the present invention has no obvious current response phenomenon for common small molecule substances ascorbic acid, uric acid, and dopamine in human blood, and has good anti-interference ability. The presence of will not affect the measurement results of the modified electrode for glucose concentration.

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Abstract

The invention discloses a preparation method of a non-enzyme glucose electrochemical sensor. The preparation method comprises the following steps: (1) pretreating a substrate; (2) preparing a mixed metal ion solution and a sulfide solution; (3) performing in-situ growth on the substrate to prepare a copper-cobalt-nickel sulfide modification layer; (4) drying the prepared copper-cobalt-nickel sulfide electrode; and (5) using the copper-cobalt-nickel composite sulfide electrode prepared in the step (4) as a working electrode, forming a three-electrode system with a counter electrode and a reference electrode, and connecting the three-electrode system with an electrochemical workstation to form an electrochemical sensor, thereby obtaining the copper-cobalt-nickel composite sulfide enzyme-free glucose electrochemical sensor. The novel non-enzyme sensor is constructed by using the copper-cobalt-nickel composite sulfide, is applied to high-sensitivity detection of the glucose content in human serum, and shows a relatively wide linear range, an extremely low detection limit and good anti-interference capability and stability.

Description

technical field [0001] The invention belongs to the field of biochemical sensors, and relates to a copper-cobalt-nickel composite sulfide enzyme-free glucose sensor and a preparation method. Background technique [0002] Diabetes has become one of the chronic diseases that seriously endanger human health. In order to prevent and monitor diabetes, it is necessary to quickly, accurately and continuously detect the content of glucose in the human body. [0003] Compared with spectrophotometry or chromatography, electrochemical detection method has the advantages of high sensitivity, fast response, easy preparation and portability. At present, glucose sensors containing enzymes are widely used in the market, but the enzymes are not only expensive, but also easily inactivated by the external environment, which seriously affects the reliability of the detection results. Therefore, the preparation of low-cost, high-sensitivity, high-stability enzyme-free glucose sensors has become...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 舒婷曾德福李宁徐伟航周亮王诗吴诗
Owner HUBEI UNIV OF SCI & TECH
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