Novel application of DHA as feed additive in regulation and control of muscle fiber types

A new use, muscle fiber technology, applied in the direction of animal feed, application, additional food elements, etc., can solve the problems of no relevant technical reports, research is still blank, etc., to increase the number of mitochondria, broaden the scope of application, and improve the quality of skeletal muscle Effect

Pending Publication Date: 2021-10-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Given that several studies have found that m 6 A can regulate skeletal muscle function, such as myogenesis, lipid deposition and muscle regeneration, while no studies have shown that DHA can affect m 6 A modification regulates skeletal muscle function
[0006] Through the above analysis, the existing problems and defects of the existing technology are: the current research on the molecular mechanism of docosahexaenoic acid regulating the transformation of muscle fiber type is still blank, and there is no relevant technical report

Method used

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  • Novel application of DHA as feed additive in regulation and control of muscle fiber types
  • Novel application of DHA as feed additive in regulation and control of muscle fiber types
  • Novel application of DHA as feed additive in regulation and control of muscle fiber types

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Daily gavage of 500 mg / kg body weight of docosahexaenoic acid to mice can increase the exercise tolerance of mice.

[0035] 1. In order to study the effect of docosahexaenoic acid on the exercise endurance of mouse skeletal muscle, 8-week-old male C57BL / 6 mice were used as the research object.

[0036] 2. Experimental method

[0037] (1) Animal feeding method: 2 animals / cage; feeding temperature and humidity: temperature is 24±1°C, humidity is 40% to 70%; 12h: 12h day and night lighting is used; feeding conditions are always kept stable to ensure the test results reliability. During the breeding period, the animals had free access to drinking water, maintenance feed (NFD) and high-fat feed (HFD) were provided by Jiangsu Synergy Biological Co., Ltd. The purchased SPF grade C57BL / 6 mice were pre-fed for 1 week to adapt to the environment.

[0038]2) Animal grouping and treatment: 8-week-old mice were randomly divided into three groups: normal diet group (NFD...

Embodiment 2

[0049] Embodiment 2: the DHA of mouse skeletal muscle mRNA mRNA m 6 Effect of A modification.

[0050] 1. In order to study the effect of DHA on the muscle fiber types of mouse skeletal muscle, 8-week-old male C57BL / 6 mice were used as the research object.

[0051] 2. Experimental method

[0052] (1) Animal breeding method: the same as in Example 1.

[0053] (2) Grouping and treatment of animals: same as in Example 1.

[0054] (3) Sampling and detection: On the day of the end of the test in step (2), mice were humanely sacrificed by cervical dislocation, gastrocnemius muscles of all mice were collected, and total mRNA was extracted for mass spectrometry detection of mRNA m 6 A level, extract protein for Western Blot detection m 6 A-related protein expression.

[0055] 3. The result is as follows Figure 5-6 Shown:

[0056] Figure 5 is the effect of DHA administration on mouse skeletal muscle m 6 Effect of A modification level.

[0057] The result is as Figure 5 As...

Embodiment 3

[0060] Example 3: Whether DHA is verified by mRNA m at the myoblast level 6 A modification regulates myofiber type switching and mitochondrial generation.

[0061] 1. In order to study the mechanism of DHA on the transformation of mouse skeletal muscle fiber types, the C2C12 cell line was used as the research object.

[0062] 2. Experimental method

[0063] (1) Cell culture and induced differentiation: C2C12 myoblasts were amplified by high-glucose DMEM complete medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin solution. When the cell density reached 90%, the culture was replaced. The base was high-glucose DMEM induction medium supplemented with 2% horse-cow serum and 1% penicillin-streptomycin solution to induce differentiation, and the amount of DHA added was 5-10 μM.

[0064] (2) Cell transfection: use the liposome transfection method to introduce the synthetic siFto small molecule interference sequence into myoblasts.

[0065] (3) Mitochondr...

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Abstract

The invention discloses novel application of DHA as a feed additive in regulation and control of muscle fiber types. According to the novel application of DHA as a feed additive, DHA is added in high-fat diet to increase mRNA m6A demethylase FTO protein expression in skeletal muscle tissue of a mouse, so the total mRNA m6A modification level in the skeletal muscle of the mouse is reduced. Under the condition of the high-fat diet, the quantity of mitochondria in the skeletal muscle of the mouse is increased, the oxidative phosphorylation gene expression of the skeletal muscle of the mouse is promoted, the glycolysis gene expression is reduced, conversion from the muscle fiber type of the skeletal muscle to slow muscle fiber is promoted, and the exercise tolerance of the mouse is improved. The addition amount of DHA in the high-fat diet is 500 mg/kg of body weight/day. DHA promotes expression of m6A demethylase FTO in a C2C12 cell line and reduces the level of m6A. DHA does not contain substances harmful to animals or human bodies, is small in addition amount, can be directly added into an animal feed, food or drugs, has the advantages of being safe, effective, free of toxic and side effects and the like, and has good popularization and application prospects in the application as the feed additive to improve the quality of animal meat.

Description

technical field [0001] The invention belongs to the field of feed additive and skeletal muscle movement. More specifically, it involves the molecular mechanism research and application of docosahexaenoic acid as a feed additive in increasing the proportion of animal slow muscle fibers, improving animal exercise endurance and meat quality. Background technique [0002] Skeletal muscle accounts for about 50% of the body weight of an adult animal, and it is composed of numerous contractile muscle fibers. According to the different metabolic characteristics of muscle fibers, muscle fibers can be divided into different types, mainly divided into slow-twitch fibers mainly based on oxidative phosphorylation metabolism mode and fast-twitch fibers mainly based on glycolysis metabolism mode. In daily life, the type of muscle fiber is closely related to the body's exercise capacity and metabolic level; while in the process of animal production, the composition and difference of muscle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/158A23K50/50
CPCA23K20/158A23K50/50
Inventor 王新霞陈炜陈雨诗汪以真
Owner ZHEJIANG UNIV
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