Check patentability & draft patents in minutes with Patsnap Eureka AI!

Two-in-one integrated determination method for analyzing circRNA-microRNA interaction

A two-in-one, complex technology, applied in the field of genes, can solve the problems of time-consuming and laborious detection procedures, high cost of RNA sequencing, failure to detect the interaction between circRNA and miRNA, etc., and achieve the effect of broadening the application range and high sensitivity

Pending Publication Date: 2021-10-08
NANJING UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method is more accurate, RNA sequencing is accompanied by high cost and professional sequencing institutions
[0004] Overall, current methods still encounter some shortcomings in practical applications: (1) usually target two related RNA molecules (circRNA and miRNA) separately to define the interaction between them; (2) cannot detect Interaction of low expressed circRNA and miRNA; (3) detection procedure is usually time-consuming and laborious
[0006] Currently, there is a lack of a highly sensitive two-in-one integrated assay for analyzing circRNA-microRNA interactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Two-in-one integrated determination method for analyzing circRNA-microRNA interaction
  • Two-in-one integrated determination method for analyzing circRNA-microRNA interaction
  • Two-in-one integrated determination method for analyzing circRNA-microRNA interaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A two-in-one integrated assay method for analyzing circRNA-microRNA interaction of the present invention comprises the following steps: (1) preparing functionalized magnetic beads (MB): the capture probe is passed between biotin and streptavidin The specific binding between immobilized on the surface of SA-MB; step1 Wash 100 μL of 10 mg / mL SA-MB twice with 1 mL of washing buffer, and then resuspend in 500 μL of biotin-streptavidin binding buffer in the liquid. Step2 Add 2 μL of 100 μM biotinylated capture probe to the SA-MBs dispersion, and then incubate the mixture on a thermomixer at 37° C. for 30 minutes. Step3 After washing 3 times with 1 mL of washing buffer to remove excess free probes, the capture probe MBs were resuspended in 100 μL of hybridization buffer for subsequent experiments, the calculation of the number of probes bound to MBs: (before reaction concentration - concentration after reaction) × volume of reaction solution.

[0034] (2) circRNA-miRNA inte...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a two-in-one integrated determination method for analyzing circRNA-microRNA interaction. The method comprises the following steps of: (1) preparing a functionalized MB: fixing a capture probe on the surface of SA-MB through specific binding between biotin and streptavidin; and (2) analyzing circRNA-microRNA interaction. The determination method disclosed by the invention is high in sensitivity, can be used for analyzing circRNA-microRNA interaction (cmRRI), and is expected to provide a useful tool for analyzing cmRRIs in an RNA dialogue system in biomedicine and clinical inventions.

Description

technical field [0001] The present invention relates to the field of gene technology, in particular to a two-in-one integrated assay for analyzing circRNA-microRNA interaction. Background technique [0002] RNA-RNA interaction (RRI) plays an important regulatory role in various basic cell life activities, for example, transfer RNA (tRNA) interacts with messenger RNA (mRNA) to translate genetic code; miRNA interacts with mRNA to promote its Degradation; Circular RNA (circRNA) interacts with miRNA by acting as a molecular sponge, which is called the sponge effect. Among them, the sponge effect is a classic model of circRNA-mediated gene regulation. Exploring the circRNA-miRNA sponge effect can not only expand the present invention's understanding of RNA interaction genomes, but also help to understand the biological functions and diseases of non-polyA circRNAs Signaling pathways involved in development. CircRNAs contain a large number of miRNA response elements (MREs), enabl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6834C12Q1/682
CPCC12Q1/6834C12Q1/682C12Q2563/143C12Q2563/149C12Q2565/519C12Q2525/207C12Q2531/125C12Q2563/107
Inventor 项阳焦瑾
Owner NANJING UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com