Low-sugar hypsizygus marmoreus mother strain culture medium containing mushroom foot powder and preparation
The technology of mother seed culture medium and mushroom mushroom feet is applied in the field of low-sugar type crab-flavored mushroom parent seed culture medium and preparation field, which can solve the problems of environmental pollution, waste of resources and the like, achieve fast growth speed, active growth metabolism, and reduce cultivation cost effect
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Embodiment 1
[0025] Embodiment 1: Low-sugar type crab-flavored mushroom mother seed culture medium and preparation method containing shiitake mushroom foot powder
[0026] (1) Weigh 20g of 1cm×1cm×1cm peeled diced potatoes into a 500mL beaker, add 80ml of tap water, heat to boiling, continue to simmer until the glass rod can squeeze the diced potatoes, filter, and place the filtrate in a 250mL beaker, Obtain potato extract.
[0027] (2) Add 1.9g of glucose, 2.0g of agar powder and 1.5g of Lentinus edodes powder to the potato extract obtained in step (1) in sequence, heat to boiling with stirring, add water to make up to 100mL, and cool.
[0028] (3) When the temperature of the culture medium drops to about 90°C, aliquot it, sterilize it at 121°C, and set aside.
[0029] Adopt the same method to prepare other less-sugar-type crab-flavored mushroom parent culture medium containing mushroom foot powder, only need to change the quality of glucose into 1.8g, 1.7g, 1.6g, 1.5g, 1.4g, 1.3g, 1.2g...
Embodiment 2
[0030] Embodiment 2: Low-sugar type crab-flavored mushroom mother seed culture medium containing crab-flavored mushroom foot powder and preparation method
[0031] (1) Weigh 20g of 1cm×1cm×1cm peeled diced potatoes into a 500mL beaker, add 80ml of tap water, heat to boiling, continue to simmer until the glass rod can squeeze the diced potatoes, filter, and place the filtrate in a 250mL beaker, Obtain potato extract.
[0032] (2) Add 1.9g of glucose, 2.0g of agar powder and 0.5g of crab-flavored mushroom foot powder to the potato extract obtained in step (1), heat to boiling with stirring, add water to make up to 100mL, and cool.
[0033] (3) When the temperature of the culture medium drops to about 90°C, aliquot it, sterilize it at 121°C, and set aside.
[0034] Use the same method to prepare other low-sugar crab-flavored mushroom parent culture medium containing crab-flavored mushroom foot powder, only need to change the quality of glucose into 1.8g, 1.7g, 1.6g, 1.5g, 1.4g, ...
Embodiment 3
[0035] Example 3: Low-sugar type crab-flavored mushroom mother seed culture medium containing white jade mushroom foot powder and its preparation method
[0036] (1) Weigh 20g of 1cm×1cm×1cm peeled diced potatoes into a 500mL beaker, add 80ml of tap water, heat to boiling, continue to simmer until the glass rod can squeeze the diced potatoes, filter, and place the filtrate in a 250mL beaker, Obtain potato extract.
[0037] (2) Add 1.9 g of glucose, 2.0 g of agar powder and 0.5 g of white jade mushroom foot powder to the potato extract obtained in step (1), heat to boiling with stirring, add water to make up to 100 mL, and cool.
[0038] (3) When the temperature of the culture medium drops to about 90°C, aliquot it, sterilize it at 121°C, and set aside.
[0039] Use the same method to prepare other low-sugar crab-flavored mushroom mother seed culture medium containing white jade mushroom foot powder, only need to change the quality of glucose into 1.8g, 1.7g, 1.6g, 1.5g, 1.4g,...
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