Biochip decoding method based on fluorescence signal intensity value

Abstract

The invention provides a biochip decoding method based on a fluorescence signal intensity value. In a one-step hybridization reaction of the decoding method, at least two tag sequences are subjected to hybridization reaction with a plurality of decoding sequences at the same time, meanwhile, the types of fluorophores of the decoding sequences are more, and in order to further improve the decoding efficiency, a multi-element logistic regression model is trained by using training data to serve as a classifier through statistical modeling. Fluorescence signal intensity is further utilized, a fluorescence signal intensity value is included in a characteristic range used when a probe is decoded, and quantitative data information of a decoding sequence emitting a fluorescence signal is analyzed and obtained. According to the technical scheme, the amount of information obtained in one decoding experiment is increased, the number of times of decoding experiments needed for decoding the same number of probe types is reduced, and therefore the decoding efficiency of decoding the multiple probes on the biochip in the production link of the biochip is greatly improved.

Description

technical field [0001] The invention relates to the technical field of biochips, in particular to a biochip decoding method based on fluorescence signal intensity values ​​in the production link. Background technique [0002] The manufacturing principle of the biochip is that first, different types of probes are connected to different batches of microbeads, and each batch of microbeads corresponds to only one kind of probe, and then all the microbeads with probes are Mix and scramble, and randomly and evenly embed the scrambled microbeads into the holes on the surface of the silicon plate to obtain a biochip. However, the biochip at this time cannot be used for subsequent detection, and it is necessary to combine the types of probes corresponding to different hole positions on the biochip, that is, the decoding file corresponding to the biochip. Therefore, only the decoded biochip can be used. Can be formally used for follow-up testing. Due to the randomness of loading mic...

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the technical defects of less information and low decoding efficiency in the one-step hybridization reaction in the biochip decoding in the prior art, the purpose of the present invention is to provide a biochip decoding method based on the fluorescence signal intensity value. The biochip includes different types of probes to be decoded, microbeads and a substrate with holes on the surface, the microbeads are embedded in the holes, and the different types of probes to be decoded have composite label sequences , the composite tag sequence includes an X-segment tag sequence that can specifically hybridize with a complementary decoding sequence at the same time, X≥2, preferably, 2≤X≤5, and in each step of the decoding experiment, the X-segment tag sequence can At most X decoding sequences can be hybridized at the same time, in other words, one or more of the X segment tag sequences can not hybridize with the decoding sequence

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