Detection method of biomolecules and inhibitor molecules

Abstract

The invention belongs to the technical field of analysis and detection, and particularly provides a detection method of biomolecules and inhibitor molecules. The detection method of biomolecules and inhibitor molecules comprises the following steps: mixing an object to be detected with a viscous solution or hydrogel to form a mixed solution, placing the mixed solution at one end of an indicating test strip, and enabling different quantities of target detection objects to cause different hydrolysis degrees of the viscous solution or the hydrogel, so that the contents of water released by the viscous solution or the hydrogel through decomposition are different, the pH sensor can move on the pH test strip at different distances, and the target detection object can be detected according to the length of the moving distance. The problems that a common biomolecule detection method in the prior art is tedious in operation steps, needs to train professionals to operate related equipment, is long in detection time and low in efficiency, causes resource waste and cost increase, and influences the detection sensitivity due to environment background factors such as matrixes exist are solved.

Description

technical field [0001] The disclosure belongs to the technical field of analysis and detection, and specifically provides a detection method for biomolecules and inhibitor molecules. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] As markers, some biomolecules can be objectively measured and used as evaluation indicators to judge normal biological processes, pathological processes or post-response responses, and are also important early warning indicators when organisms are damaged or diseased. For this reason, experimenters often need to perform biomolecular detection when exploring pathology or other biological research. [0004] At present, the commonly used detection methods mainly include enzyme-linked immunoassay, chemiluminescence immunoassay and electrochemical analysis. Usually, these methods have high selectivity and specificity, but ...

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Problems solved by technology

[0005] For the biomolecular detection methods commonly used in the prior art, the operation steps are cumbersome, and professionals need to be trained to operate related equipment, the detection time is long and the efficiency is low, resulting in waste of resources and increased costs. At the same time, there are environmental background factors such as substrates that affect the detection sensitivity. Problem, in one or some embodiments of the present disclosure, a detection method of biomolecules is provided, comprising the following steps: mixing the analyte with a viscous solution (such as polymer solution, hydrogel, etc.) to form a mixed solution, and placing At one end of the indicator test strip, different amounts of target substances cause different degrees of hydrolysis of the components of the viscous solution, so the content of the water released by the decomposition of the viscous solution or hydrogel is different, resulting in different distances on the pH test strip. Move, realize the detection of the target detection object through the length of the moving distance

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