Extract for enhancing immunity and delaying senescence and preparation method thereof

A technology that enhances immunity and delays aging. It is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, and the functions of food ingredients. Effect

Inactive Publication Date: 2021-10-29
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the health foods with anti-oxidation and anti-aging effects use Western medicine analysis, reduction and confrontation methods to ac

Method used

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  • Extract for enhancing immunity and delaying senescence and preparation method thereof
  • Extract for enhancing immunity and delaying senescence and preparation method thereof
  • Extract for enhancing immunity and delaying senescence and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] A preparation method of anti-aging extract:

[0037] S1. Cleaning and soaking: Use ultrasonic waves to clean 500 parts of antlers (sika deer, red deer or deer antlers off the plate) and 250 parts of tortoise shells (carapace and plastron of turtles of the turtle family), and then completely soak the antlers for 30 days. Ultrasonic cleaning and water change once a day;

[0038] S2. Initial boiling: boil the soaked antlers with purified water at a ratio of 1:20, drain water after the first boiling for 10-30 minutes, then add water to cover the antlers, keep the temperature at 90-100°C, and add cinnamon 10 parts were boiled at constant temperature for 48 hours, and the extract was concentrated and collected;

[0039] S3. Cook again: continue to boil the antlers with purified water, then keep the temperature at 100°C, add 10 parts of cinnamon, and boil at a constant temperature for 48 hours. The extract is concentrated and collected, and then the water is changed;

[0040...

Embodiment

[0045] Take 10mL of the extract obtained by adding resistant dextrin to the enzymolysis solution.

experiment example 1

[0048] The sensory evaluation table of table 1 embodiment and comparative example

[0049]

[0050] In summary, the sensory evaluation of the extract by the method described in the examples is the best (95 points).

[0051] 2. Anti-aging activity evaluation

[0052] 2.1 Test sample

[0053] Uracil-deficient Escherichia coli (op50), Examples 1-3 and positive control (64uM astaxanthin standard):

[0054] After the extract was dissolved in water, samples of 0.5, 2.5, and 5.0 (mg / mL) were mixed with op50 for intervention; the test substance in the blank group only contained op50; the astaxanthin samples in the positive control group were dissolved in dimethyl sulfoxide (DMSO) Afterwards, a 64uM sample was prepared with op50 for intervention.

[0055] 2.2 Synchronization of C. elegans

[0056] Synchronization of C. elegans by bleaching. Select most of the NGM culture dishes of nematodes in the egg-laying stage, wash them into EP tubes with M9 buffer solution, add the lysate...

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PUM

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Abstract

The invention discloses an extract for enhancing immunity and delaying senescence, and belongs to the technical field of functional foods. The extract comprises the following raw material components: deer horn, tortoise shell, purified water, cinnamon, protease, American ginseng, medlar and resistant dextrin. The extract selects the American ginseng and the cinnamon, is sweet and pleasant in taste, and has the effects of nourishing yin and warming and invigorating kidney yang; tortoise shell glue and deer horn glue have the effect of prolonging life in traditional Chinese pharmacology; and the tortoise shell has the effect of improving the immune function, can effectively prolong the life of caenorhabditis elegans, reduces the ROS and MDA levels in vivo, improves the activity of antioxidant enzymes, and has a good anti-aging effect and a great development prospect.

Description

technical field [0001] The invention belongs to the technical field of functional food, and relates to an extract for enhancing immunity and delaying aging and a preparation method thereof. Background technique [0002] Tortoise shell has the functions of nourishing yin and suppressing yang, nourishing kidney and strengthening bones, nourishing blood and nourishing the heart, consolidating meridians and stopping collapse, and can reduce the function of thyroid and adrenal cortex in rats with hyperthyroidism, improve cellular immunity and blood immunity, and delay aging; deer antlers have Warming and invigorating kidney yang, generating essence and blood, strengthening muscles and bones, and anti-inflammatory effects; Liver and kidney, extinguishing liver wind, benefiting essence and eyesight, nourishing yin and qi, nourishing muscles and bones, removing fatigue, nourishing kidney and strengthening body, regulating immunity, nourishing liver and improving eyesight; Dispellin...

Claims

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Application Information

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IPC IPC(8): A61K36/815A23L33/10A23L33/105A23L33/21A23L29/00A61P37/04A61P39/06A61K35/32A61K35/586
CPCA61K36/258A61K36/54A61K35/586A61K35/32A61K36/815A61P39/06A61P37/04A23L33/10A23L33/105A23L29/06A23L33/21A23V2002/00A61K2300/00A23V2200/324A23V2200/302A23V2250/21A23V2250/204A23V2250/5114
Inventor 黎攀陈宏著杜冰徐雅囡黄达荣邓新宇
Owner SOUTH CHINA AGRI UNIV
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