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[0019] 1. N-((4-hydroxycarbamoyl)benzyl)-1-(3,4,5-trimethoxyphenyl)-9H-pyrido[3,4-b]indole-3- Preparation and purity identification of carboxamide
[0020] N-((4-Hydroxycarbamoyl)benzyl)-1-(3,4,5-trimethoxyphenyl)-9H-pyrido[3,4-b]indole-3-carboxamide The preparation process steps are as follows: Dissolve L-tryptophan (5g, 24.5mmol) in acetic acid (50mL), heat to 90°C and stir for 20min, then add 3,4,5-methoxy-benzaldehyde (4.9g, 25.0 mmol) for 6h. After the reaction was finished, the pH was adjusted to neutral with 2M NaOH, and filtered by suction to obtain Compound 3 as a white solid.
[0021] Compound 3 (5g, 13.2mmol) was dissolved in DMF (10ml), cooled in ice, potassium permanganate (4.172g, 26.4mmol) was added, and reacted at room temperature for 1 hour. After the reaction was finished, filter with suction, add the remaining solution into water, and filter with suction to obtain compound 4.
[0022] Compound 4 (0.45g, 1.2mM), EDCI (0.27g, 1.44mM), DMAP (17mg, 0.14mM), ...
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